Kim Sang-Hyun, Shin Tae-Yong
CMRI, IHBR, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
Toxicol In Vitro. 2009 Oct;23(7):1215-9. doi: 10.1016/j.tiv.2009.07.036. Epub 2009 Aug 7.
Mast cell-mediated allergic inflammation is involved in many diseases such as asthma and sinusitis. Mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 with immune regulatory properties. In the present study, we investigate the effect an unspecified aqueous extract from leaves of Eriobotrya japonica Lindl. (Rosaceae) (LEJL) on the expression of pro-inflammatory cytokines and its possible mechanisms of action in human mast cells (HMC-1). LEJL dose-dependently inhibited phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187 (PMACI)-induced gene expression and secretion of TNF-alpha, IL-6, and IL-8. LEJL attenuated PMACI-induced activation of nuclear factor (NF)-kappaB, and specifically blocked activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) but not that of c-jun N-terminal kinase. The inhibitory effect of LEJL on the pro-inflammatory cytokines was likely NF-kappaB, p38 MAPK, and ERK dependent. Our in vitro studies provide evidence that LEJL might contribute to the treatment of mast cell-derived allergic inflammatory diseases.
肥大细胞介导的过敏性炎症涉及许多疾病，如哮喘和鼻窦炎。肥大细胞诱导合成和产生具有免疫调节特性的促炎细胞因子，包括肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和IL-8。在本研究中，我们调查了一种未指明的枇杷叶水提取物（LEJL）对人肥大细胞（HMC-1）中促炎细胞因子表达的影响及其可能的作用机制。LEJL剂量依赖性地抑制佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）和钙离子载体A23187（PMACI）诱导的TNF-α、IL-6和IL-8的基因表达和分泌。LEJL减弱了PMACI诱导的核因子（NF）-κB的激活，并特异性地阻断了p38丝裂原活化蛋白激酶（MAPK）和细胞外信号调节激酶（ERK）的激活，但不影响c-jun氨基末端激酶的激活。LEJL对促炎细胞因子的抑制作用可能依赖于NF-κB、p38 MAPK和ERK。我们的体外研究提供了证据，表明LEJL可能有助于治疗肥大细胞源性过敏性炎症疾病。
