Singh A, Singh S, Kanungo M S
Department of Zoology, Banaras Hindu University, Varanasi, India.
Mol Biol Rep. 1990 Nov;14(4):251-4. doi: 10.1007/BF00429893.
The conformation and expression of the albumin gene in the liver of young (21-) and old (85-week) rats were studied. Digestion of nuclei with MNase shows no differences in the nucleosomal organization in the coding region of the gene in the two ages. The gene has a DNase I hypersensitive site which is distinctly less sensitive in old rats. Its 5'-CCGG-3' sequences are more methylated in the old in which its rate of transcription is also lower.
研究了幼年（21周龄）和老年（85周龄）大鼠肝脏中白蛋白基因的构象和表达。用微球菌核酸酶消化细胞核表明，该基因编码区域的核小体组织在两个年龄段没有差异。该基因有一个脱氧核糖核酸酶I超敏感位点，在老年大鼠中其敏感性明显降低。其5'-CCGG-3'序列在老年大鼠中甲基化程度更高，其转录速率也更低。
