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从乌干达的自然蚊虫种群中分离出一种新型黄病毒和库蚊黄病毒(黄病毒科)的一个新毒株。

Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda.

作者信息

Cook Shelley, Moureau Gregory, Harbach Ralph E, Mukwaya Louis, Goodger Kim, Ssenfuka Fred, Gould Ernest, Holmes Edward C, de Lamballerie Xavier

机构信息

Natural History Museum, Cromwell Road, London SW7 5BD, UK.

Unité des Virus Emergents UMR190 'Emergence des Pathologies Virales', Université de la Méditerranée et Institut de Recherche pour le Développement, Marseille, France.

出版信息

J Gen Virol. 2009 Nov;90(Pt 11):2669-2678. doi: 10.1099/vir.0.014183-0. Epub 2009 Aug 5.

Abstract

The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known 'insect-only' flavivirus, appearing to replicate in insect cells alone. A second member of the 'insect-only' group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the 'insect-only' group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel 'insect-only' flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa.

摘要

黄病毒属包含约70种单链正义RNA病毒,是研究虫媒疾病进化的独特模型,因为它包括蚊媒病毒、蜱媒病毒或尚无已知传播媒介的病毒。理论研究和实地研究均表明存在大量未被发现的黄病毒。最近,从波多黎各的自然蚊虫种群中首次分离出细胞融合剂病毒(CFAV),并且在泰国的蚊虫中发现了与CFAV相关的序列。CFAV此前于1975年从蚊细胞系中分离出来,是唯一已知的“仅在昆虫中存在”的黄病毒,似乎仅在昆虫细胞中复制。“仅在昆虫中存在”的病毒组的第二个成员,卡米蒂河病毒(KRV),于2003年从肯尼亚蚊虫中分离出来。“仅在昆虫中存在”的病毒组的第三个暂定成员,库蚊黄病毒(CxFV),于2007年首次从日本分离出来,随后在美洲报道了更多毒株。我们报告了从乌干达恩德培发现、分离和鉴定的两种新型“仅在昆虫中存在”的黄病毒:一个暂定命名为纳基沃戈病毒(NAKV)的新谱系和一株新的CxFV毒株。这些毒株所分离自的个体蚊虫,通过使用该地区凭证标本构建的参考分子系统发育树进行回顾性鉴定,分别为非洲曼蚊黑腹亚种和致倦库蚊。据我们所知,这是首次从曼蚊属物种中分离出新型的仅在昆虫中存在的黄病毒,也是首次从非洲分离出CxFV毒株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef90/2885038/461fa5a30453/2669fig1.jpg

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