快速测定野外采集蚊子中的病毒RNA序列。
Rapid determination of viral RNA sequences in mosquitoes collected in the field.
作者信息
Kihara Yuki, Satho Tomomitsu, Eshita Yuki, Sakai Kouji, Kotaki Akira, Takasaki Tomohiko, Rongsriyam Yupha, Komalamisra Narumon, Srisawat Raweewan, Lapcharoen Parichat, Sumroiphon Suchada, Iwanaga Shiroh, Ushijima Hiroshi, Endoh Daiji, Miyata Takeshi, Sakata Akira, Kashige Nobuhiro, Miake Fumio, Fukushi Shuetsu, Saijo Masayuki, Kurane Ichiro, Morikawa Shigeru, Mizutani Tetsuya
机构信息
Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
出版信息
J Virol Methods. 2007 Dec;146(1-2):372-4. doi: 10.1016/j.jviromet.2007.07.008. Epub 2007 Sep 17.
A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses.
一种快速测定病毒RNA序列（RDV）的方法应用于从泰国登革热（登革出血热）流行地区采集的埃及伊蚊匀浆，采用蚊细胞系C6/36。检测到了4型登革病毒和细胞融合剂病毒的核酸序列。这种RDV方法有可能成为检测已知和新出现的未知蚊媒病毒的标准方法。
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