Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan; Department of Medical Research (Pyin Oo Lwin Branch), Ministry of Health and Sports, Myanmar.
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
Virus Res. 2018 Mar 2;247:120-124. doi: 10.1016/j.virusres.2018.01.007. Epub 2018 Feb 4.
An entomological surveillance of arboviruses was conducted in Myanmar in 2014. A total of 8357 Culex mosquito vectors were collected in the Mandalay area and virus isolation was done by using the mosquito cell line C6/36 E2. A total of eighteen strains of Culex flavivirus (CxFV) were isolated from Cx. tritaeniorhynchus, Cx. vishnui and Cx. fuscocephala. Like other insect-specific flaviviruses, CxFV can replicate only in mosquito cells but not in mammalian cells. These CxFV strains that were isolated in Japan from mosquitoes collected in Myanmar were closely related to the Wang Thong virus detected from Cx fusocephalus in Thailand and Cx.theileri flavivirus (CTFV) isolated from Cx. theileri mosquitoes in Portugal and Turkey. They encode a single open reading frame with 3357 amino acid residues. They have the characteristics of flaviviruses and have 95.62% amino acid identity with CTFV. This is the first report of CxFV in Myanmar with the characterized viral genome. This study illustrated that CxFV was circulating among the vectors of human pathogenic arboviruses in Myanmar but the impact of CxFV on other flaviviruses which are endemic in the study area still remains to be explored.
2014 年在缅甸开展了虫媒病毒病的昆虫学监测。在曼德勒地区共采集了 8357 只库蚊蚊虫作为媒介，并使用 C6/36 E2 蚊细胞株进行病毒分离。从三带喙库蚊、白纹伊蚊和褐头库蚊中分离到 18 株黄病毒属蚊虫病毒（CxFV）。与其他昆虫特异性黄病毒一样，CxFV 只能在蚊细胞中复制，而不能在哺乳动物细胞中复制。这些在日本从缅甸采集的蚊虫中分离到的 CxFV 株与在泰国从褐头库蚊中检测到的 Wang Thong 病毒以及从葡萄牙和土耳其分离到的 Cx. theileri 黄病毒（CTFV）密切相关。它们编码一个带有 3357 个氨基酸残基的单一开放阅读框。它们具有黄病毒的特征，与 CTFV 的氨基酸同一性为 95.62%。这是缅甸首次报道 CxFV，并对其病毒基因组进行了特征描述。本研究表明，CxFV 在缅甸的人类致病虫媒病毒媒介中传播，但 CxFV 对该研究地区流行的其他黄病毒的影响仍有待进一步研究。
