School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand.
Reprod Sci. 2009 Dec;16(12):1144-52. doi: 10.1177/1933719109342756. Epub 2009 Aug 5.
To date, there is no reliable test to identify women in early pregnancy at risk of developing preeclampsia. Difference gel electrophoresis (DIGE) identified the plasma proteins vitronectin (VN) and high-molecular-weight kininogen (HK) in association with preeclampsia. In a longitudinal proteomics study, the plasma of preeclamptic patients (n = 6) was compared to healthy control participants (n = 6) before the onset of preeclampsia (week 20) and at the time of presentation with clinical disease (weeks 33-36). The 75-kd single-chain VN molecule increased 1.6- to 1.9-fold in preeclampsia, whereas the 65-kd moiety of the 2-chain VN molecule decreased 1.5- to 1.7-fold compared to healthy controls (P < .05). Immunoblots revealed differences in proteolytic processing of VN and/or HK in women who develop preeclampsia or preeclampsia further complicated by small-for-gestational-age. Vitronectin and HK may prove to be useful as early markers of fibrinolytic activity and neutrophil activation, which are known to be associated with preeclampsia.
迄今为止，尚无可靠的测试方法可用于识别处于妊娠早期且有先兆子痫风险的女性。差异凝胶电泳（DIGE）鉴定了与先兆子痫相关的血浆蛋白（VN）和高分子量激肽原（HK）。在一项纵向蛋白质组学研究中，在先兆子痫发作之前（第 20 周）和出现临床疾病时（第 33-36 周），将先兆子痫患者（n=6）的血浆与健康对照组（n=6）进行了比较。75-kd 单链 VN 分子在先兆子痫中增加了 1.6-1.9 倍，而 2 链 VN 分子的 65-kd 部分与健康对照组相比降低了 1.5-1.7 倍（P<0.05）。免疫印迹揭示了在发生先兆子痫或因胎儿生长受限而使先兆子痫进一步复杂化的女性中，VN 和/或 HK 的蛋白水解加工存在差异。VN 和 HK 可能被证明是有用的纤溶活性和中性粒细胞活化的早期标志物，已知这与先兆子痫有关。
