Teixeira Vitor H, Valente Hugo F, Casal Susana I, Marques A Franklim, Moreira Pedro A
Faculty of Nutrition and Food Sciences, University of Porto, Porto, Potugal.
Med Sci Sports Exerc. 2009 Sep;41(9):1752-60. doi: 10.1249/MSS.0b013e31819fe8e3.
This study aimed to determine the effects of 4 wk of antioxidants (AOX) supplementation on exercise-induced lipid peroxidation, muscle damage, and inflammation in kayakers.
Subjects (n = 20) were randomly assigned to receive a placebo (PLA) or an AOX capsule (AOX; 272 mg of alpha-tocopherol, 400 mg of vitamin C, 30 mg of beta-carotene, 2 mg of lutein, 400 mug of selenium, 30 mg of zinc, and 600 mg of magnesium). Blood samples were collected at rest and 15 min after a 1000-m kayak race, both before and after the supplementation period, for analysis of alpha-tocopherol, alpha-carotene, beta-carotene, lycopene, lutein plus zeaxanthin, vitamin C, uric acid, total AOX status (TAS), thiobarbituric reactive acid substances (TBARS) and interleukin-6 (IL-6) levels, and creatine kinase (CK), superoxide dismutase (SOD), glutathione reductase (Gr), and glutathione peroxidase (GPx) activities.
With supplementation, plasma alpha-tocopherol (P = 0.003) and beta-carotene (P = 0.007) augmented significantly in the AOX group. IL-6 (exercise, P = 0.039), TBARS (exercise, P < 0.001), and uric acid (exercise, P = 0.032) increased significantly in response to the exercise regardless of treatment group. Cortisol level raised more from pre- to postsupplementation period in the PLA group (time x supplementation, P = 0.002). Although TAS declined after exercise before intervention, it increased above preexercise values after the 4-wk period in the AOX group (supplementation x time x exercise, P = 0.034). CK increased after exercise in both groups (exercise effect, P < 0.001) and decreased from week 0 to week 4 more markedly in the PLA group (supplementation x time, P = 0.049).
AOX supplementation does not offer protection against exercise-induced lipid peroxidation and inflammation and may hinder the recovery of muscle damage.
本研究旨在确定补充4周抗氧化剂（AOX）对皮划艇运动员运动诱导的脂质过氧化、肌肉损伤和炎症的影响。
将受试者（n = 20）随机分为两组，分别接受安慰剂（PLA）或AOX胶囊（AOX；含272毫克α-生育酚、400毫克维生素C、30毫克β-胡萝卜素、2毫克叶黄素、400微克硒、30毫克锌和600毫克镁）。在补充期前后，于静息状态以及1000米皮划艇比赛后15分钟采集血样，分析α-生育酚、α-胡萝卜素、β-胡萝卜素、番茄红素、叶黄素加玉米黄质、维生素C、尿酸、总抗氧化状态（TAS）、硫代巴比妥酸反应性酸物质（TBARS）和白细胞介素-6（IL-6）水平，以及肌酸激酶（CK）、超氧化物歧化酶（SOD）、谷胱甘肽还原酶（Gr）和谷胱甘肽过氧化物酶（GPx）活性。
补充后，AOX组血浆α-生育酚（P = 0.003）和β-胡萝卜素（P = 0.007）显著增加。无论治疗组如何，运动后IL-6（运动，P = 0.039）、TBARS（运动，P < 0.001）和尿酸（运动，P = 0.032）均显著增加。PLA组从补充前到补充后的皮质醇水平升高更多（时间×补充，P = 0.002）。尽管干预前运动后TAS下降，但AOX组在4周后增加至高于运动前值（补充×时间×运动，P = 0.034）。两组运动后CK均升高（运动效应，P < 0.001），且PLA组从第0周到第4周下降更明显（补充×时间，P = 0.049）。
补充AOX不能预防运动诱导的脂质过氧化和炎症，且可能阻碍肌肉损伤的恢复。
