Stein Erich W, Maslov Konstantin, Wang Lihong V
J Appl Phys. 2009 May 15;105(10):102027. doi: 10.1063/1.3116134. Epub 2009 May 19.
Noninvasive, high resolution imaging of mouse brain activity is poised to provide clinically translatable insights into human neurological disease progression. Toward noninvasive imaging of brain activity through the hemodynamic response, the dark-field photoacoustic microscopy (PAM) technique was enhanced to image the cortex vasculature of the mouse brain in vivo using endogenous hemoglobin contrast. Specifically, the PAM system was redesigned to efficiently collect photoacoustic waves originating from cortical vessels, providing high (70 mum lateral and 54 mum axial) resolution images of the mouse brain vasculature with a contrast-to-noise ratio of 25 dB. These findings confirm the efficacy of PAM to noninvasively image vascular structures in the mouse brain and the potential to image mouse brain function by tracking the hemodynamic response.
对小鼠大脑活动进行无创、高分辨率成像，有望为人类神经疾病进展提供具有临床可转化性的见解。为了通过血流动力学响应实现大脑活动的无创成像，暗场光声显微镜（PAM）技术得到改进，利用内源性血红蛋白对比剂对小鼠大脑皮层血管进行体内成像。具体而言，对PAM系统进行了重新设计，以有效收集源自皮层血管的光声波，提供小鼠脑血管系统的高分辨率（横向70微米、轴向54微米）图像，对比度噪声比为25分贝。这些发现证实了PAM对小鼠大脑血管结构进行无创成像的有效性，以及通过追踪血流动力学响应来成像小鼠大脑功能的潜力。
