Zhang Hao F, Maslov Konstantin, Wang Lihong V
Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
Nat Protoc. 2007;2(4):797-804. doi: 10.1038/nprot.2007.108.
Functional photoacoustic microscopy (fPAM) is a hybrid technology that permits noninvasive imaging of the optical absorption contrast in subcutaneous biological tissues. fPAM uses a focused ultrasonic transducer to detect high-frequency photoacoustic (PA) signals. Volumetric images of biological tissues can be formed by two-dimensional raster scanning, and functional parameters can be further extracted from spectral measurements. fPAM is safe and applicable to animals as well as humans. This protocol provides guidelines for parameter selection, system alignment, imaging operation, laser safety and data processing for in vivo fPAM. It currently takes approximately 100 min to carry out this protocol, including approximately 50 min for data acquisition using a 10-Hz pulse-repetition-rate laser system. The data acquisition time, however, can be significantly reduced by using a laser system with a higher pulse repetition rate.
功能光声显微镜（fPAM）是一种混合技术，可对皮下生物组织中的光吸收对比度进行无创成像。fPAM使用聚焦超声换能器来检测高频光声（PA）信号。通过二维光栅扫描可以形成生物组织的体积图像，并且可以从光谱测量中进一步提取功能参数。fPAM安全且适用于动物和人类。本方案提供了体内fPAM的参数选择、系统校准、成像操作、激光安全和数据处理的指导方针。目前执行本方案大约需要100分钟，包括使用10 Hz脉冲重复率激光系统进行数据采集的大约50分钟。然而，通过使用具有更高脉冲重复率的激光系统，可以显著减少数据采集时间。
