Koroleva G A, Lashkevich V A, Voroshilova M K
Arch Virol. 1977;54(1-2):29-39. doi: 10.1007/BF01314376.
Multiplication of virulent and vaccine strains of poliovirus type I, II and III in laboratory animals of different species was studied comparatively. The main criterion of virus reproduction was the production of the photoresistant virus progeny after inoculation of the animals with proflavin-photosensitized virus strains. On the whole, virulent poliovirus strains were characterized by replication in a wide range of hosts (monkeys, cotton rats, white mice, guinea pigs, rabbits, chickens, chick embryos), a low infective dose, production of the photoresistant progeny to a high titre, clinically overt disease in some animal species. The vaccine strains multiplied in a norrower range of hosts, had a high infective dose, a low titre of virus progeny, and caused no clinical symptoms of infection. These differences may serve as a marker for differentiation between virulent and attenuated strains in vivo. Administration of guanidine before inoculation of newborn cotton rats completely prevented or delayed by several days the production of photoresistant virus progeny. This fact confirms the stability of the proflavin-poliovirus complex under conditions ruling out virus replication.
对Ⅰ型、Ⅱ型和Ⅲ型脊髓灰质炎病毒的强毒株和疫苗株在不同物种实验动物中的增殖情况进行了比较研究。病毒繁殖的主要标准是在用原黄素 - 光致敏病毒株接种动物后产生抗光病毒后代。总体而言，强毒株脊髓灰质炎病毒的特征是在广泛的宿主（猴子、棉鼠、小白鼠、豚鼠、兔子、鸡、鸡胚）中复制，感染剂量低，产生高滴度的抗光后代，在某些动物物种中出现明显的临床疾病。疫苗株在较窄的宿主范围内增殖，感染剂量高，病毒后代滴度低，且不引起感染的临床症状。这些差异可作为体内区分强毒株和减毒株的标志物。在接种新生棉鼠之前给予胍可完全预防或使抗光病毒后代的产生延迟数天。这一事实证实了原黄素 - 脊髓灰质炎病毒复合物在排除病毒复制的条件下的稳定性。
