Balcar V J
Department of Anatomy, University of Sydney, N.S.W., Australia.
Neurochem Res. 1990 Dec;15(12):1237-8. doi: 10.1007/BF01208585.
[3H]GABA at low concentrations (5-10 nM) was bound by what appeared to be a "GABA receptor binding site" in bacterial contamination originating from a batch of distilled water. Under experimental conditions similar to those usually employed in [3H]GABA binding studies, the apparent binding displayed a very high "specific" component and a high efficiency in terms of [3H]GABA bound per mg of protein. The "binding" was blocked by muscimol but not by isoguvacine, SR95531 and nipecotic acid. These characteristics suggest that the presence of such spurious binding in the experiments using 3H-labeled ligands in brain homogenates may not always be very obvious and, moreover, it can result in subtle, but serious, distortions of data from such studies, which may not be immediately recognized.
低浓度（5 - 10纳摩尔）的[³H]GABA与一批蒸馏水中细菌污染物中一个看似“GABA受体结合位点”的物质结合。在与通常用于[³H]GABA结合研究的实验条件相似的情况下，这种表观结合表现出非常高的“特异性”成分，并且就每毫克蛋白质结合的[³H]GABA而言效率很高。这种“结合”可被蝇蕈醇阻断，但不能被异鹅去甲肾上腺素、SR95531和哌啶甲酸阻断。这些特征表明，在脑匀浆中使用³H标记配体的实验中，这种虚假结合的存在可能并不总是很明显，而且，它可能会导致此类研究数据出现细微但严重的扭曲，而这些扭曲可能不会立即被识别出来。
