Sheng Zhiyong, Fu Xiaobing, Cai Sa, Lei Yonghong, Sun Tongzhu, Bai Xiaodong, Chen Minliang
Burns Institute, The First Affiliated Hospital, Chinese PLA General Hospital, Trauma Center of Postgraduate Medical College, Beijing 10048, China.
Wound Repair Regen. 2009 May-Jun;17(3):427-35. doi: 10.1111/j.1524-475X.2009.00474.x.
Regeneration of sweat glands after deep burns has been an unsolved problem. Owing to lack of perspiration, survivors of an extensive deep burn injury are leading a miserable life in sultry months. It was our contemplation to solve this problem by inducing bone marrow mesenchymal stem cells (MSCs) to acquire the phenotype of sweat gland cells in vitro. Then these cells were transplanted into fresh skin wounds resulting from excision of anhydrotic scars after healing of deep burn injury in five patients. Two to 12 months after the procedure, it was proved that there was recovery of perspiration function in all the MSCs' transplanted areas, as evidenced by positive iodine-starch perspiration test. Histological and biochemical observation confirmed the involvement of MSCs transformed sweat gland cells in the recovery of functional sweat glands, and the components of sweat collected from these areas were similar to that collected from normal skin. This is the first report of successful transplantation of MSCs in regenerating functional sweat glands, which may help solve the problem of depletion of sweat glands in patients surviving extensive deep burns in the future.
深度烧伤后汗腺的再生一直是个未解决的问题。由于缺乏排汗功能，大面积深度烧伤的幸存者在闷热的月份里过着痛苦的生活。我们设想通过在体外诱导骨髓间充质干细胞（MSCs）获得汗腺细胞表型来解决这个问题。然后将这些细胞移植到5例深度烧伤愈合后切除无汗瘢痕所形成的新鲜皮肤伤口处。术后2至12个月，碘淀粉发汗试验阳性证明所有MSC移植区域的排汗功能均得以恢复。组织学和生化观察证实，转化为汗腺细胞的MSCs参与了功能性汗腺的恢复，且从这些区域收集的汗液成分与从正常皮肤收集的汗液成分相似。这是关于MSCs成功移植再生功能性汗腺的首次报道，这可能有助于解决未来大面积深度烧伤幸存者汗腺缺失的问题。
