Pore Debasis, Chowdhury Pinki, Mahata Nibedita, Pal Amit, Yamasaki Shinji, Mahalanabis Dilip, Chakrabarti Manoj K
Division of Pathophysiology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India.
Vaccine. 2009 Sep 25;27(42):5855-64. doi: 10.1016/j.vaccine.2009.07.054. Epub 2009 Aug 4.
In the present study we purified 34 kDa major outer membrane protein (MOMP) of Shigella flexneri 2a for the first time, which was cross-reactive and antigenically conserved among Shigella spp. and the epitope was surface exposed on the intact bacterium. The purified antigen was found to be glycosylated, which aids in binding to macrophages and up-regulated the production of nitric oxide, granulocyte-colony stimulating factor and IL-12p70, indicating that the MOMP is immunogenic and has the ability to commence protective immune responses against intracellular pathogens, thereby it may be considered as a potential vaccine candidate.
在本研究中，我们首次纯化了福氏志贺菌2a的34 kDa主要外膜蛋白（MOMP），该蛋白在志贺菌属中具有交叉反应性且抗原保守，其表位暴露于完整细菌的表面。发现纯化的抗原是糖基化的，这有助于与巨噬细胞结合并上调一氧化氮、粒细胞集落刺激因子和IL-12p70的产生，表明MOMP具有免疫原性，并有能力启动针对细胞内病原体的保护性免疫反应，因此它可能被视为一种潜在的疫苗候选物。
