将DNA折叠成扭曲和弯曲的纳米级形状。

Folding DNA into twisted and curved nanoscale shapes.

作者信息

Dietz Hendrik, Douglas Shawn M, Shih William M

机构信息

Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.

出版信息

Science. 2009 Aug 7;325(5941):725-30. doi: 10.1126/science.1174251.

Abstract

We demonstrate the ability to engineer complex shapes that twist and curve at the nanoscale from DNA. Through programmable self-assembly, strands of DNA are directed to form a custom-shaped bundle of tightly cross-linked double helices, arrayed in parallel to their helical axes. Targeted insertions and deletions of base pairs cause the DNA bundles to develop twist of either handedness or to curve. The degree of curvature could be quantitatively controlled, and a radius of curvature as tight as 6 nanometers was achieved. We also combined multiple curved elements to build several different types of intricate nanostructures, such as a wireframe beach ball or square-toothed gears.

摘要

我们展示了利用DNA在纳米尺度上构建可扭曲和弯曲的复杂形状的能力。通过可编程的自组装,DNA链被引导形成紧密交联的双螺旋定制形状束,这些双螺旋与它们的螺旋轴平行排列。碱基对的靶向插入和缺失会导致DNA束产生左旋或右旋扭曲,或者弯曲。弯曲程度可以定量控制,并且实现了低至6纳米的曲率半径。我们还将多个弯曲元件组合起来构建了几种不同类型的复杂纳米结构,如线框沙滩球或方齿齿轮。

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