Instituto de la Vid y del Vino de Castilla-La Mancha, Crta. Toledo-Albacete s/n, 13700, Tomelloso (Ciudad Real), Spain.
J Biosci Bioeng. 2009 Sep;108(3):220-4. doi: 10.1016/j.jbiosc.2009.04.001.
The genetic diversity of 339 strains of lactic acid bacteria responsible for the spontaneous malolactic fermentation (MLF) in Tempranillo wines produced in six cellars located in different provinces of Castilla-La Mancha region of Spain was analyzed by using randomly amplified polymorphic DNA (RAPD). The results indicate that Oenococcus oeni was the predominant species in MLF of Tempranillo wines with a scant diversity of other lactic acid bacteria (LAB) species. The presence of a well-adapted cellar microbiota is confirmed, with MLF dominated by a limited number of O. oeni genotypes. Clustering of isolates from samples taken from different provinces showed the existence of coincident genotypes in different cellars of the region. This result suggests that a population of cosmopolitan O. oeni strains exists in the Castilla-La Mancha wine-growing region.
采用随机扩增多态性 DNA（RAPD）技术分析了西班牙卡斯蒂利亚-拉曼恰（Castilla-La Mancha）地区 6 个不同酒庄酿造的添普兰尼洛（Tempranillo）葡萄酒中 339 株自发苹果酸-乳酸发酵（MLF）乳酸菌的遗传多样性。结果表明，葡萄酒中 MLF 主要由片球菌属（Oenococcus oeni）主导，其他乳酸菌（LAB）的多样性较少。这证实了适应性良好的酒窖微生物群的存在，MLF 主要由少数 O. oeni 基因型主导。对来自不同省份的样本进行分离物聚类分析表明，该地区不同酒窖存在一致的基因型。这一结果表明，在卡斯蒂利亚-拉曼恰葡萄酒产区存在一个具有世界性分布的 O. oeni 菌株种群。
