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用问号钩端螺旋体LigA重组片段与载体分子结合免疫仓鼠后的免疫反应。

Immune response in hamsters immunised with a recombinant fragment of LigA from Leptospira interrogans, associated with carrier molecules.

作者信息

Oliveira Thaís L, Bacelo Kátia L, Schuch Rodrigo A, Seixas Fabiana K, Collares Tiago, Rodrigues Oscar Ed, Vargas Josimar, Nascimento Rafaella O do, Dellagostin Odir A, Hartwig Daiane D

机构信息

Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.

Universidade Federal de Santa Maria, Departamento de Química, Santa Maria, RS, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2016 Nov;111(11):712-716. doi: 10.1590/0074-02760160214. Epub 2016 Oct 13.

DOI:10.1590/0074-02760160214
PMID:27759768
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5125051/
Abstract

Immunisation with the C-terminal region of leptospiral immunoglobulin-like A protein (LigANI) has shown promising results against leptospirosis. We evaluated the humoral immune response and protection induced by LigANI associated with carboxyl multi-walled carbon nanotubes (COOH-MWCNTs), CpG oligodeoxynucleotides (CpG ODNs), or Alhydrogel. Animals immunised with CpG ODNs were unable to develop a humoral immune response, whereas immunisation with LigANI and COOH-MWCNTs produced a high level of IgG antibodies, similar to that with LigANI and Alhydrogel, but it was not protective. The use of carbon nanotubes as an adjuvant in subunit vaccines against leptospirosis is a novel approach for improving specific IgG production.

摘要

用钩端螺旋体免疫球蛋白样A蛋白（LigANI）的C端区域进行免疫接种已显示出抗钩端螺旋体病的良好效果。我们评估了与羧基多壁碳纳米管（COOH-MWCNTs）、CpG寡脱氧核苷酸（CpG ODNs）或氢氧化铝佐剂相关的LigANI诱导的体液免疫反应和保护作用。用CpG ODNs免疫的动物无法产生体液免疫反应，而用LigANI和COOH-MWCNTs免疫则产生了高水平的IgG抗体，与用LigANI和氢氧化铝佐剂免疫的情况相似，但没有保护作用。将碳纳米管用作抗钩端螺旋体病亚单位疫苗的佐剂是提高特异性IgG产生的一种新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/6618ee55182d/0074-0276-mioc-0074-02760160214-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/1796f9cc9233/0074-0276-mioc-0074-02760160214-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/0824e63b8b21/0074-0276-mioc-0074-02760160214-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/afa743c87011/0074-0276-mioc-0074-02760160214-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/6618ee55182d/0074-0276-mioc-0074-02760160214-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/1796f9cc9233/0074-0276-mioc-0074-02760160214-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/0824e63b8b21/0074-0276-mioc-0074-02760160214-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/afa743c87011/0074-0276-mioc-0074-02760160214-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d6/5125051/6618ee55182d/0074-0276-mioc-0074-02760160214-gf04.jpg

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Vaccines against leptospirosis.
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