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使用埃洛石粘土和羧基功能化多壁碳纳米管进行重组LipL32抗原递送可增强IgG反应。

The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response.

作者信息

Hartwig Daiane D, Bacelo Kátia L, Oliveira Thaís L, Schuch Rodrigo, Seixas Fabiana K, Collares Tiago, Rodrigues Oscar, Hartleben Cláudia P, Dellagostin Odir A

机构信息

Centro de Desenvolvimento Tecnológico.

Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2015 Feb;110(1):134-7. doi: 10.1590/0074-02760140276. Epub 2015 Jan 23.

DOI:10.1590/0074-02760140276
PMID:25742273
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4371227/
Abstract

We studied the feasibility of using halloysite clay nanotubes (HNTs) and carboxyl-functionalised multi-walled carbon nanotubes (COOH-MWCNTs) as antigen carriers to improve immune responses against a recombinant LipL32 protein (rLipL32). Immunisation using the HNTs or COOH-MWCNTs significantly increased the rLipL32-specific IgG antibody titres (p < 0.05) of Golden Syrian hamsters. None of the vaccines tested conferred protection against a challenge using a virulent Leptospira interrogans strain. These results demonstrated that nanotubes can be used as antigen carriers for delivery in hosts and the induction of a humoral immune response against purified leptospiral antigens used in subunit vaccine preparations.

摘要

我们研究了使用埃洛石粘土纳米管（HNTs）和羧基功能化多壁碳纳米管（COOH-MWCNTs）作为抗原载体来增强针对重组LipL32蛋白（rLipL32）的免疫反应的可行性。使用HNTs或COOH-MWCNTs进行免疫显著提高了金黄叙利亚仓鼠的rLipL32特异性IgG抗体滴度（p<0.05）。所测试的疫苗均未对强毒问号钩端螺旋体菌株的攻击提供保护。这些结果表明，纳米管可用作抗原载体，用于在宿主体内递送，并诱导针对亚单位疫苗制剂中使用的纯化钩端螺旋体抗原的体液免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed6/4371227/f9d6f390f7e0/0074-0276-mioc-110-01-00134-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed6/4371227/f9d6f390f7e0/0074-0276-mioc-110-01-00134-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed6/4371227/f9d6f390f7e0/0074-0276-mioc-110-01-00134-gf01.jpg

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本文引用的文献

1
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Ther Deliv. 2011 Aug;2(8):1057-77. doi: 10.4155/tde.11.68.
2
Single-walled carbon nanotubes deliver peptide antigen into dendritic cells and enhance IgG responses to tumor-associated antigens.单壁碳纳米管将肽抗原递送入树突状细胞，并增强针对肿瘤相关抗原的 IgG 应答。
ACS Nano. 2011 Jul 26;5(7):5300-11. doi: 10.1021/nn200182x. Epub 2011 Jun 23.
3
Structural and proactive safety aspects of oxidation debris from multiwalled carbon nanotubes.
LigA formulated in AS04 or Montanide ISA720VG induced superior immune response compared to alum, which correlated to protective efficacy in a hamster model of leptospirosis.
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Front Immunol. 2022 Oct 10;13:985802. doi: 10.3389/fimmu.2022.985802. eCollection 2022.
4
Nanoclays: Promising Materials for Vaccinology.纳米黏土：疫苗学的有前景材料。
Vaccines (Basel). 2022 Sep 17;10(9):1549. doi: 10.3390/vaccines10091549.
5
Application of Halloysite Nanotubes in Cancer Therapy-A Review.埃洛石纳米管在癌症治疗中的应用——综述
Materials (Basel). 2021 May 29;14(11):2943. doi: 10.3390/ma14112943.
6
DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection.使用 LemA 抗原制备的 DNA 纳米疫苗可保护金黄地鼠免受钩端螺旋体致死性感染。
Mem Inst Oswaldo Cruz. 2020 Apr 17;115:e190396. doi: 10.1590/0074-02760190396. eCollection 2020.
7
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多壁碳纳米管氧化残骸的结构和主动安全性方面。
J Hazard Mater. 2011 May 15;189(1-2):391-6. doi: 10.1016/j.jhazmat.2011.02.050. Epub 2011 Feb 23.
4
Characterization of the immunogenic and antigenic potential of putative lipoproteins from Leptospira interrogans.鉴定问号钩端螺旋体可能的脂蛋白的免疫原性和抗原性。
Curr Microbiol. 2011 Apr;62(4):1337-41. doi: 10.1007/s00284-010-9865-1. Epub 2011 Jan 11.
5
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J Phys Chem B. 2010 Apr 29;114(16):5625-31. doi: 10.1021/jp100903x.
6
Cytocompatibility and uptake of halloysite clay nanotubes.海泡石纳米管的细胞相容性和摄取。
Biomacromolecules. 2010 Mar 8;11(3):820-6. doi: 10.1021/bm9014446.
7
Immunological and cytotoxicological characterization of tuberculin purified protein derivative (PPD) conjugated to single-walled carbon nanotubes.结核菌素纯化蛋白衍生物（PPD）与单壁碳纳米管偶联的免疫原性和细胞毒性特征。
Immunol Lett. 2009 Sep 22;126(1-2):48-53. doi: 10.1016/j.imlet.2009.07.012. Epub 2009 Aug 5.
8
Leptospira and leptospirosis.钩端螺旋体与钩端螺旋体病。
Vet Microbiol. 2010 Jan 27;140(3-4):287-96. doi: 10.1016/j.vetmic.2009.03.012. Epub 2009 Mar 13.
9
Halloysite clay nanotubes for controlled release of protective agents.用于保护剂控释的埃洛石粘土纳米管。
ACS Nano. 2008 May;2(5):814-20. doi: 10.1021/nn800259q.
10
Impact of nanotechnology on drug delivery.纳米技术对药物递送的影响。
ACS Nano. 2009 Jan 27;3(1):16-20. doi: 10.1021/nn900002m.