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三种血清学技术用于诊断牛1型疱疹病毒的比较：血清中和试验、酶联免疫吸附测定和间接免疫荧光法。

Comparison of three serological techniques for the diagnosis of bovine herpesvirus type 1: serum neutralization, enzyme-linked immunosorbent assay and indirect immunofluorescence.

作者信息

Bratanich A, Sardi S, Smitsaart E, Estevez Madero J, Schudel A A

机构信息

Instituto de Virología, INTA Castelar, Buenos Aires, Argentina.

出版信息

Rev Argent Microbiol. 1990 Oct-Dec;22(4):192-8.

PMID:1966502
Abstract

Serum neutralization (SN), indirect immunofluorescence (IFI) and enzyme-linked immunosorbent assay (ELISA) were compared for their sensitivity and specificity to detect bovine serum antibodies to Bovine Herpesvirus type 1 on 105 bovine serum samples. High correlation coefficients among all three techniques were observed, showing a higher sensitivity for the ELISA test. Due to its simplicity and its feasibility to be adopted in less equipped laboratories, the ELISA test is recommended for large scale epizotiological studies, serological diagnosis and detection of reactive animals.

摘要

对105份牛血清样本，比较了血清中和试验（SN）、间接免疫荧光试验（IFI）和酶联免疫吸附测定（ELISA）检测牛1型疱疹病毒血清抗体的敏感性和特异性。观察到所有三种技术之间具有较高的相关系数，ELISA试验显示出更高的敏感性。由于ELISA试验操作简单且可在设备较差的实验室采用，因此推荐用于大规模流行病学研究、血清学诊断和反应性动物的检测。

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