Rosskopf M, Staub E, Ackermann M
Institut für Virologie, Veterinärmedizinische Fakultät, Universität Zürich.
Schweiz Arch Tierheilkd. 1994;136(2):58-67.
Sensitivity and specificity of two ELISA systems for the detection of antibodies to Bovine Herpes-virus 1 (BHV1; IBR/IPV) were tested by using 214 sera of cattle with predetermined history of BHV1 infection and freedom from BHV1 infection, respectively. With these sera as "gold-standard", the sensitivity of ELISA1 (HerdCheck: Anti-IBR) appeared to exceed 98%, whereas for ELISA2 (Checkit: Trachitest) a sensitivity of between 94 and 99.3% was determined. The specificity of ELISA1 amounted to at least 96.9%, whereas for ELISA2 it ranged apparently from 81.5 to 98.5%. When applied in routine testings of serum samples, the two ELISA systems correlated extremely well. With each test, only 3 of 1431 samples gave contradictory results. In all these cases, alternative tests suggested that the contradictions represented false-positive reactions. A similar correlation was observed when milk samples in the place of sera were probed. The serum and milk samples were additionally tested using two corresponding ELISA systems (obtained from the same manufacturers) for the detection of antibodies to Bovine Leukemia virus (BLV). The sensitivity and specificity of these tests could not be determined because of lack of samples with known history of infection. The results of the tests, however, correlated very well. Only 6 of 1431 sera reacted in a contradictory way. These observations indicate that the kits tested in this study, both for the detection of antibodies to BHV1 and to BLV, meet high quality standards. Possibilities to improve the kits were still detected.
使用214份分别具有牛疱疹病毒1型（BHV1；传染性牛鼻气管炎/传染性脓疱性外阴阴道炎，IBR/IPV）预定感染史和未感染史的牛血清，对两种用于检测抗BHV1抗体的酶联免疫吸附测定（ELISA）系统的敏感性和特异性进行了测试。以这些血清作为“金标准”，ELISA1（HerdCheck：抗IBR）的敏感性似乎超过98%，而ELISA2（Checkit：Trachitest）的敏感性测定为94%至99.3%。ELISA1的特异性至少为96.9%，而ELISA2的特异性明显在81.5%至98.5%之间。当应用于血清样本的常规检测时，这两种ELISA系统的相关性非常好。每次检测中，1431份样本中只有3份给出了矛盾的结果。在所有这些情况下，其他检测表明这些矛盾代表假阳性反应。当用牛奶样本代替血清进行检测时，也观察到了类似的相关性。此外，使用两种相应的ELISA系统（从同一制造商处获得）对血清和牛奶样本进行检测，以检测抗牛白血病病毒（BLV）抗体。由于缺乏已知感染史的样本，无法确定这些检测的敏感性和特异性。然而，检测结果的相关性非常好。1431份血清中只有6份反应相互矛盾。这些观察结果表明，本研究中测试的试剂盒，无论是用于检测抗BHV1抗体还是抗BLV抗体，都符合高质量标准。不过，仍发现了改进试剂盒的可能性。
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