Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1113-1117. doi: 10.1099/ijs.0.014795-0. Epub 2009 Aug 7.
A Gram-negative-staining, aerobic, non-motile and rod-shaped bacterial strain, S5-5(T), was isolated from a tidal flat sediment at Saemankum on the west coast of Korea and subjected to a polyphasic taxonomic investigation. Strain S5-5(T) grew optimally at pH 7.5-8.0, at 30 degrees C and in the presence of 2 % (w/v) NaCl. It did not produce bacteriochlorophyll a. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain S5-5(T) is phylogenetically closely related to the genus Roseivivax, joining the cluster comprising the two recognized Roseivivax species. The 16S rRNA gene sequence similarity between strain S5-5(T) and members of the genus Roseivivax was in the range 95.0-96.7 %. Strain S5-5(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)omega7c and 11-methyl C(18 : 1)omega7c as the major fatty acids. The DNA G+C content was 68.2 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain S5-5(T) could be differentiated from Roseivivax species. On the basis of the data presented, strain S5-5(T) is considered to represent a novel species of the genus Roseivivax, for which the name Roseivivax lentus sp. nov. is proposed. The type strain is S5-5(T) (=KCTC 22708(T) =CCUG 57755(T)).
一株革兰氏阴性、需氧、非运动、杆状的细菌菌株 S5-5(T)，从韩国西海岸咸安郡的潮滩沉积物中分离出来，并进行了多相分类学研究。菌株 S5-5(T)在 pH7.5-8.0、30°C 和 2%（w/v）NaCl 存在下生长最佳。它不产生细菌叶绿素 a。基于 16S rRNA 基因序列的系统发育分析表明，菌株 S5-5(T)与 Roseivivax 属在系统发育上密切相关，加入了由两个公认的 Roseivivax 种组成的聚类。菌株 S5-5(T)与 Roseivivax 属成员之间的 16S rRNA 基因序列相似性在 95.0-96.7%之间。菌株 S5-5(T)含有 Q-10 作为主要的泛醌和 C(18:1)ω7c 和 11-甲基 C(18:1)ω7c 作为主要的脂肪酸。DNA G+C 含量为 68.2mol%。差异表型特性加上系统发育的独特性表明，菌株 S5-5(T)可以与 Roseivivax 种区分开来。基于所提出的数据，菌株 S5-5(T)被认为代表了 Roseivivax 属的一个新种，建议使用 Roseivivax lentus sp. nov.这个名称。模式菌株为 S5-5(T)=KCTC 22708(T)=CCUG 57755(T)。
