Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Jun;64(Pt 6):1958-1963. doi: 10.1099/ijs.0.062190-0. Epub 2014 Mar 10.
A Gram-stain-negative, non-motile, ovoid- or rod-shaped bacterial strain, designated S-22(T), belonging to the class Alphaproteobacteria, was isolated from a tidal flat sediment of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain S-22(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2-3% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain S-22(T) joined Wenxinia marina HY34(T), with which it exhibited highest 16S rRNA gene sequence similarity (98.0%). The DNA G+C content was 72.3 mol% and the mean DNA-DNA relatedness value between strain S-22(T) and the type strain of W. marina was 3.7%. Strain S-22(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6c) and C(16 : 0) as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain S-22(T) is distinguishable from W. marina. On the basis of the data presented, strain S-22(T) is considered to represent a novel species of the genus Wenxinia, for which the name Wenxinia saemankumensis sp. nov. is proposed. The type strain of is S-22(T) ( = KCTC 32548(T) = CECT 8456(T)).
一株革兰氏阴性、非运动性、卵形或杆状的细菌菌株，被命名为 S-22(T)，属于α变形菌纲，从韩国黄海潮滩沉积物中分离出来，并进行了多相分类学研究。菌株 S-22(T) 在 pH7.0-8.0、25°C 和 2-3%（w/v）NaCl 存在下生长最佳。基于 16S rRNA 基因序列的邻接法、最大似然法和最大简约法系统发育树显示，菌株 S-22(T)与 Wenxinia marina HY34(T)聚在一起，两者的 16S rRNA 基因序列相似度最高（98.0%）。DNA G+C 含量为 72.3 mol%，菌株 S-22(T)与 W. marina 模式菌株的 DNA-DNA 同源性平均值为 3.7%。菌株 S-22(T)含有 Q-10 作为主要的泛醌，特征脂肪酸为 summed feature 8（C(18 : 1)ω7c 和/或 C(18 : 1)ω6c）和 C(16 : 0)。主要的极性脂为磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、一种未鉴定的氨基脂、一种未鉴定的糖脂、一种未鉴定的磷脂和一种未鉴定的脂。表型特征的差异，以及系统发育和遗传上的独特性，表明菌株 S-22(T)与 W. marina 不同。基于所提供的数据，菌株 S-22(T)被认为代表 Wenxinia 属的一个新种，因此提议将其命名为Wenxinia saemankumensis sp. nov.。其模式菌株为 S-22(T)（ = KCTC 32548(T) = CECT 8456(T)）。
