Dos Santos Rogério Lacerda, Pithon Matheus Melo, Da Silva Mendes Gabriella, Romanos Maria Teresa Villela, De Oliveira Ruellas Antônio Carlos
Federal University of Alfenas, Alfenas, MG, Brazil.
J Appl Oral Sci. 2009 Jul-Aug;17(4):326-9. doi: 10.1590/s1678-77572009000400010.
Natural latex does not fall into the category of materials known to be entirely inoffensive. The purpose of this in vitro study was to test the hypothesis that there is no difference in the cytotoxicity between elastics of different colors and those from different manufacturers.
Different latex intraoral elastics of different colors (5/16 = 7.9 mm, mean load) were compared. The sample was divided into 7 groups of 24 elastics each: Group T (TP Orthodontics, natural latex elastics, control); Groups U1, U2, U3, U4, U5 and U6 (Uniden, natural latex elastics and colored elastics, namely, green, pink, yellow, red and purple, respectively). Cytotoxicity assays were performed by using cell culture medium containing epithelioid-type cells (Hep-2 line) derived from human laryngeal carcinoma. The cytotoxicity was evaluated by using the 'dye-uptake' test, which was employed at two different moments (0 and 24 h). Data were compared by analysis of variance (ANOVA) and Tukey's test (p<0.05).
There was statistically significant difference (p<0.05) between Group T and all other groups (U1, U2, U3, U4, U5 and U6) at 0 and 24 h. No statistically significant difference (p<0.05) was found between Groups U1 and U5, U1 and U6, U2 and U3, U2 and U4, U2 and U5, U2 and U6, U3 and U4, U3 and U5, U3 and U6, U4 and U5, U4 and U6, and U5 and U6 at 0 and 24 h.
The TP Orthodontics elastics promoted less cell lysis compared to the Uniden elastics regardless of their color.
天然乳胶不属于已知完全无害的材料类别。本体外研究的目的是检验不同颜色和不同制造商生产的弹力线之间细胞毒性无差异这一假设。
比较不同颜色（5/16 = 7.9毫米，平均负荷）的不同乳胶口腔弹力线。样本分为7组，每组24根弹力线：T组（TP正畸公司，天然乳胶弹力线，对照）；U1、U2、U3、U4、U5和U6组（Uniden公司，天然乳胶弹力线和彩色弹力线，分别为绿色、粉色、黄色、红色和紫色）。使用含有源自人喉癌的上皮样细胞（Hep - 2系）的细胞培养基进行细胞毒性测定。通过“染料摄取”试验在两个不同时间点（0和24小时）评估细胞毒性。数据通过方差分析（ANOVA）和Tukey检验进行比较（p<0.05）。
在0和24小时时，T组与所有其他组（U1、U2、U3、U4、U5和U6）之间存在统计学显著差异（p<0.05）。在0和24小时时，U1与U5、U1与U6、U2与U3、U2与U4、U2与U5、U2与U6、U3与U4、U3与U5、U3与U6、U4与U5以及U4与U6以及U5与U6之间未发现统计学显著差异（p<0.05）。
无论颜色如何，与Uniden弹力线相比，TP正畸公司的弹力线导致的细胞裂解较少。
