Umemura Kazuo, Noguchi Yusuke, Ichinose Takuya, Hirose Yo, Kuroda Reiko, Mayama Shigeki
Musashi Institute of Technology, Setagaya, Tokyo 158-8557, Japan.
J Biol Phys. 2008 Apr;34(1-2):189-96. doi: 10.1007/s10867-008-9086-z. Epub 2008 Jun 24.
We demonstrate the cultivation of diatom cells on a functionalized mica surface and the preparation of frustules on a mica surface by baking. Diatom cells were successfully grown on a mica surface treated with 3-aminopropyltriethoxysilane. After baking at 400 degrees C for 2 h, frustule structures without the organic components of the diatom cells were successfully observed by scanning electron microscopy and atomic force microscopy. Furthermore, the frustules deformed and became slender when a sample was baked at 800 degrees C for 2 h. Our method is effective for the direct characterization of frustule structures and physical properties without changing the configuration of the diatom cells grown on the mica surface.
我们展示了在功能化云母表面培养硅藻细胞以及通过烘烤在云母表面制备硅藻壳的过程。硅藻细胞成功地生长在经3-氨丙基三乙氧基硅烷处理的云母表面。在400℃烘烤2小时后，通过扫描电子显微镜和原子力显微镜成功观察到不含硅藻细胞有机成分的硅藻壳结构。此外，当样品在800℃烘烤2小时时，硅藻壳会变形并变细长。我们的方法对于直接表征硅藻壳结构和物理性质有效，且不会改变生长在云母表面的硅藻细胞的形态。
