Suh Se Ryung, Lee Joo Hun, Go Gyung Min, Lee Jaeyoung, Kong Hyunjoon, Park Eun-Jin
Department of Prosthodontics, Graduate School of Clinical Dentistry, Ewha Womans University, Seoul 07985, Republic of Korea.
Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Materials (Basel). 2024 Nov 23;17(23):5742. doi: 10.3390/ma17235742.
High-temperature baking is a typical method to remove organic matter from diatoms, but it is not suitable for bio-silica because of the high crystallinity. This study provides a method using the VAUS to remove organic matter from diatoms more quickly and biocompatibly. The optimal frequency for organic matter removal was investigated for domestically produced . The removal efficiency of TOC/TN was calculated and analyzed. The C and Si elements were analyzed in EDS, and organic matter removal was analyzed by XRD. TOC RE% at a frequency of 35 kHz exhibited the highest value, indicating a statistically significant difference. XRD analysis demonstrated that the organic matter was nearly entirely removed using NaOCl compared to high-temperature-baked . In the EDS analysis, there were significant differences in the C and Si elements with respect to frequency. This is very similar to the values from the positive control group, high-temperature-baked . Ultrasonic treatment and frequency adjustments were found to significantly impact the chemical removal of organic matter from . Although vacuum application was initially considered, it did not demonstrate a statistically significant effect according to TOC analysis.
高温烘焙是从硅藻中去除有机物的一种典型方法，但由于结晶度高，它不适用于生物二氧化硅。本研究提供了一种使用VAUS更快且生物相容性更好地从硅藻中去除有机物的方法。研究了国产硅藻去除有机物的最佳频率。计算并分析了TOC/TN的去除效率。在能谱仪（EDS）中分析了碳（C）和硅（Si）元素，并通过X射线衍射（XRD）分析了有机物的去除情况。35kHz频率下的总有机碳去除率（TOC RE%）显示出最高值，表明存在统计学上的显著差异。XRD分析表明，与高温烘焙的硅藻相比，使用次氯酸钠（NaOCl）几乎完全去除了有机物。在EDS分析中，碳和硅元素在频率方面存在显著差异。这与阳性对照组（高温烘焙的硅藻）的值非常相似。发现超声处理和频率调整对从硅藻中化学去除有机物有显著影响。尽管最初考虑了施加真空，但根据TOC分析，它并未显示出统计学上的显著效果。
