Suh Se Ryung, Lee Joo Hun, Go Gyung Min, Lee Jaeyoung, Kong Hyunjoon, Park Eun-Jin
Ewha Womans University.
University of Illinois.
Res Sq. 2024 Aug 2:rs.3.rs-4709095. doi: 10.21203/rs.3.rs-4709095/v1.
High-temperature baking is a typical method to remove organic matter from diatoms, but it's not suitable for bio-silica because of the high crystallinity. This study provides a method using the VAUS to remove organic matter from diatoms more quickly and biocompatibly. Organic matter was removed by using VAUS, while NaOCl was utilized to remove the organic matter from the silicate frustule. The optimal frequency for organic matter removal was investigated to domestically produced The removal efficiency of TOC/TN was calculated and analyzed. The C and Si elements were analyzed in EDS, while visual confirmation of organic matter removal was analyzed by using XRD. TOC RE% at a frequency of 35kHz exhibited the highest value, indicating a statistically significant difference. XRD analysis demonstrated that the organic matter was almost removed using NaOCl compared to the high-temperature baked . In the EDS analysis, there were significant differences in the C and Si elements with respect to frequency. This is very similar to the values from the positive control group, high-temperature baked This new procedure of applying periodic negative pressure to NaOCl pre-treatment is considered to be an effective method of chemically removing organic matter from diatoms.
高温烘焙是从硅藻中去除有机物的一种典型方法，但由于其高结晶度，它不适用于生物二氧化硅。本研究提供了一种使用可变幅度超声（VAUS）更快且具有生物相容性地从硅藻中去除有机物的方法。使用VAUS去除有机物，同时使用次氯酸钠（NaOCl）从硅酸盐壳中去除有机物。研究了国产的用于去除有机物的最佳频率。计算并分析了总有机碳/总氮（TOC/TN）的去除效率。在能谱仪（EDS）中分析了碳（C）和硅（Si）元素，同时使用X射线衍射仪（XRD）对有机物去除进行了直观确认。在35kHz频率下的总有机碳去除率（TOC RE%）显示出最高值，表明存在统计学上的显著差异。XRD分析表明，与高温烘焙的相比，使用NaOCl几乎可以去除有机物。在EDS分析中，碳和硅元素在频率方面存在显著差异。这与阳性对照组（高温烘焙的）的值非常相似。这种对NaOCl预处理施加周期性负压的新方法被认为是从硅藻中化学去除有机物的有效方法。
