M. D. Anderson Cancer Center, The University of Texas, Houston, TX 77030, USA.
Biomed Microdevices. 2010 Aug;12(4):597-606. doi: 10.1007/s10544-009-9340-6.
The development of improved methods for targeted cell detection is of general interest in many fields of research and drug development. There are a number of well-established techniques for the study and detection of biomarkers expressed in living cells and tissues. Many of them rely on multi-step procedures that might not meet ideal assay requirements for speed, cost, sensitivity, and specificity. Here we report and further validate an approach that enables spontaneous molecular assembly to generate biologically active networks of bacteriophage (phage) assembled with gold (Au) nanoparticles (termed Au-phage nanoshuttles). Here, the nanoshuttles preserve the cell binding and internalization attributes mediated by a displayed peptide targeted to a cell surface receptor. The organization of such targeted assemblies can be further manipulated to be used as a multimodal detection assembly, and they can be characterized as fractal nanostructures by angle-dependent light scattering fractal dimension analysis. Targeted Au-phage nanoshuttles offer multiple functionalities for nanotechnology-based sensing and reporting, including enhanced fluorescence and improved contrast for darkfield microscopy.
开发改进的靶向细胞检测方法在许多研究和药物开发领域都具有普遍意义。目前有许多成熟的技术可用于研究和检测活细胞和组织中表达的生物标志物。其中许多技术依赖于多步程序，这些程序可能不符合速度、成本、灵敏度和特异性等理想测定要求。在这里，我们报告并进一步验证了一种方法，该方法能够通过自发分子组装生成带有金（Au）纳米粒子的噬菌体（噬菌体）组装的生物活性网络（称为 Au-噬菌体纳米穿梭）。在这里，纳米穿梭保留了由靶向细胞表面受体的肽介导的细胞结合和内化特性。这种靶向组装的组织可以进一步被操纵，以用作多模态检测组装，并且可以通过角度依赖的光散射分形维数分析将其表征为分形纳米结构。靶向 Au-噬菌体纳米穿梭为基于纳米技术的传感和报告提供了多种功能，包括增强荧光和改善暗场显微镜的对比度。
