Souza Glauco R, Christianson Dawn R, Staquicini Fernanda I, Ozawa Michael G, Snyder Evan Y, Sidman Richard L, Miller J Houston, Arap Wadih, Pasqualini Renata
University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1215-20. doi: 10.1073/pnas.0509739103. Epub 2006 Jan 24.
Biological molecular assemblies are excellent models for the development of nanoengineered systems with desirable biomedical properties. Here we report an approach for fabrication of spontaneous, biologically active molecular networks consisting of bacteriophage (phage) directly assembled with gold (Au) nanoparticles (termed Au-phage). We show that when the phage are engineered so that each phage particle displays a peptide, such networks preserve the cell surface receptor binding and internalization attributes of the displayed peptide. The spontaneous organization of these targeted networks can be manipulated further by incorporation of imidazole (Au-phage-imid), which induces changes in fractal structure and near-infrared optical properties. The networks can be used as labels for enhanced fluorescence and dark-field microscopy, surface-enhanced Raman scattering detection, and near-infrared photon-to-heat conversion. Together, the physical and biological features within these targeted networks offer convenient multifunctional integration within a single entity with potential for nanotechnology-based biomedical applications.
生物分子组装体是开发具有理想生物医学特性的纳米工程系统的优秀模型。在此，我们报告了一种制备由噬菌体（phage）与金（Au）纳米颗粒直接组装而成的自发、生物活性分子网络（称为Au-phage）的方法。我们表明，当对噬菌体进行工程改造，使每个噬菌体颗粒展示一种肽时，此类网络保留了所展示肽的细胞表面受体结合和内化特性。通过掺入咪唑（Au-phage-imid）可进一步操控这些靶向网络的自发组织，这会引起分形结构和近红外光学特性的变化。这些网络可用作增强荧光和暗场显微镜、表面增强拉曼散射检测以及近红外光子到热转换的标记物。总之，这些靶向网络中的物理和生物学特性为在单个实体中实现便捷的多功能整合提供了可能，具有基于纳米技术的生物医学应用潜力。
