Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Spain.
NMR Biomed. 2010 Jan;23(1):23-33. doi: 10.1002/nbm.1421.
MR spectroscopic Imaging (MRSI), with PRESS localization, is used here to monitor the effects of acute hyperglycemia in the spectral pattern of 11 mice bearing GL261 gliomas at normothermia (36.5-37.5 degrees C) and at hypothermia (28.5-29.5 degrees C). These in vivo studies were complemented by ex vivo high resolution magic angle spinning (HR-MAS) analysis of GL261 tumor samples from 6 animals sacrificed by focused microwave irradiation, and blood glucose measurements in 12 control mice. Apparent glucose levels, monitored by in vivo MRSI in brain tumors during acute hyperglycemia, rose to an average of 1.6-fold during hypothermia (p < 0.05), while no significant changes were detected at normothermia, or in control experiments performed at euglycemia, or in normal/peritumoral brain regions. Ex vivo analysis of glioma-bearing mouse brains at hypothermia revealed higher glucose increases in distinct regions during the acute hyperglycemic challenge (up to 6.6-fold at the tumor center), in agreement with maximal in vivo blood glucose changes (5-fold). Phantom studies on taurine plus glucose containing solutions explained the differences between in vivo and ex vivo measurements. Our results also indicate brain tumor heterogeneity in the four animal tumors investigated in response to a defined metabolic challenge.
磁共振波谱成像（MRSI）结合 PRESS 定位，用于监测在正常体温（36.5-37.5°C）和低温（28.5-29.5°C）下 11 只携带 GL261 神经胶质瘤的老鼠的光谱模式中急性高血糖的影响。这些体内研究通过聚焦微波辐射处死的 6 只动物的 GL261 肿瘤样本的离体高分辨率魔角旋转（HR-MAS）分析以及 12 只对照鼠的血糖测量进行了补充。在急性高血糖期间通过体内 MRSI 监测的脑肿瘤中的葡萄糖水平在低温下平均升高了 1.6 倍（p < 0.05），而在正常体温或对照实验（在正常血糖或正常/肿瘤周围脑区）中没有检测到显著变化。在低温下对携带神经胶质瘤的老鼠大脑进行离体分析显示，在急性高血糖挑战期间，特定区域的葡萄糖增加更高（在肿瘤中心高达 6.6 倍），与最大的体内血糖变化（5 倍）一致。含牛磺酸和葡萄糖的溶液的体模研究解释了体内和离体测量之间的差异。我们的结果还表明，在针对特定代谢挑战的四个动物肿瘤中，脑肿瘤存在异质性。
