Yang A J, Mulligan R M
Department of Developmental and Cell Biology, University of California, Irvine 92717.
Mol Cell Biol. 1991 Aug;11(8):4278-81. doi: 10.1128/mcb.11.8.4278-4281.1991.
Eighteen cytidines are changed to uridines in the coding sequence of transcripts for cytochrome c oxidase subunit 2 (cox2) in maize mitochondria. The temporal relationship of editing and splicing was examined in cox2 transcripts by sequence analysis of spliced and unspliced cDNAs. Cloned cDNAs of unspliced cox2 transcripts ranged from clones with no edited nucleotides to completely edited forms, while spliced cDNAs were nearly completely edited. Incompletely edited transcripts in the nascent pool of unspliced transcripts represent intermediates of the editing process. These results indicate that editing proceeds without a strong directional bias and suggest that RNA editing is a posttranscriptional process.
在玉米线粒体中，细胞色素c氧化酶亚基2（cox2）转录本的编码序列中有18个胞嘧啶被转变为尿嘧啶。通过对剪接和未剪接的cDNA进行序列分析，研究了cox2转录本中编辑和剪接的时间关系。未剪接的cox2转录本的克隆cDNA范围从没有编辑核苷酸的克隆到完全编辑的形式，而剪接后的cDNA几乎完全被编辑。未剪接转录本新生池中不完全编辑的转录本代表编辑过程的中间体。这些结果表明编辑过程没有强烈的方向性偏好，并表明RNA编辑是一个转录后过程。
