Nakato Gaku, Fukuda Shinji, Hase Koji, Goitsuka Ryo, Cooper Max D, Ohno Hiroshi
International Graduate School of Arts and Sciences, Yokohama City University, Kanagawa 230-0045, Japan.
DNA Res. 2009 Aug;16(4):227-35. doi: 10.1093/dnares/dsp013.
A minor population of M cells within the follicle-associated epithelium (FAE) of intestinal Peyer's patches (PPs) serves as a major portal for entry of exogenous antigens. Characterization of the mammalian M cells, including identification of M-cell surface molecules used for bacterial uptake, has been hampered by their relative rarity. In contrast, M cells constitute virtually all of the FAE cells in the avian bursa of Fabricius. We therefore performed comparative gene expression profiling of chicken and murine FAE to identify commonly expressed genes by M cells in both species. The comprehensive transcriptome analysis revealed that 28 genes were commonly up-regulated in FAE from both species. In situ hybridization revealed that annexin A10 (Anxa10) mRNA was scattered in FAE, and co-localized with Ulex europaeus agglutinin-1 binding to M cells. Whole-mount immunostaining also revealed that cellular prion protein (PrP(C)) was expressed on the luminal side of the apical plasma membrane of M cells, and co-localized with grycoprotein 2 that recognizes only M cells in murine PP. Our findings identify new M-cell-specific molecules through using comprehensive transcriptome analysis. These conserved molecules in M cells of mice and chickens may play essential roles in M-cell function and/or differentiation.
肠道派尔集合淋巴结（PPs）的滤泡相关上皮（FAE）中的一小部分M细胞是外源性抗原进入的主要门户。哺乳动物M细胞的特性，包括用于细菌摄取的M细胞表面分子的鉴定，因其相对稀少而受到阻碍。相比之下，M细胞几乎构成了禽类法氏囊的所有FAE细胞。因此，我们对鸡和小鼠的FAE进行了比较基因表达谱分析，以确定这两个物种中M细胞共同表达的基因。全面的转录组分析表明，两个物种的FAE中有28个基因共同上调。原位杂交显示，膜联蛋白A10（Anxa10）mRNA散布在FAE中，并与欧洲荆豆凝集素-1结合的M细胞共定位。整装免疫染色还显示，细胞朊蛋白（PrP(C)）在M细胞顶端质膜的腔侧表达，并与仅识别小鼠PP中M细胞的糖蛋白2共定位。我们的研究结果通过全面的转录组分析确定了新的M细胞特异性分子。小鼠和鸡的M细胞中这些保守分子可能在M细胞功能和/或分化中起重要作用。
