病例报告：由玻璃化分裂胚胎发育而来的人类再次玻璃化第7天自然孵化囊胚移植后成功分娩。
Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos.
作者信息
Hiraoka Kenichiro, Hiraoka Kaori, Horiuchi Toshitaka, Kusuda Tomoyo, Okano Shinichiro, Kinutani Masayuki, Kinutani Kazuo
机构信息
Kinutani Women's Clinic, Naka-ku, Hiroshima 730-0035, Japan.
出版信息
J Assist Reprod Genet. 2009 Jul;26(7):405-9. doi: 10.1007/s10815-009-9333-7. Epub 2009 Aug 13.
PURPOSE
To report a successful delivery after the transfer of a re-cryopreserved day-7 hatched blastocyst.
METHODS AND RESULTS
A 30-year-old woman underwent a long-treatment protocol for ovarian stimulation. Fourteen mature oocytes were obtained, and twelve were fertilized. On day 3, two cleaved embryos were transferred, but no implantation occurred. The remaining embryos were vitrified. Subsequently, two vitrified day-3 embryos were transferred. The woman became pregnant and delivered a healthy baby. Subsequently, two vitrified day-3 embryos were transferred, but no pregnancy occurred. Subsequently, all the remaining vitrified day-3 embryos were cultured. On day 5, no blastocyst was obtained. The remaining embryos were continued to be cultured. On day 7, a hatched blastocyst was obtained and re-vitrified. Subsequently, the re-vitrified day-7 hatched blastocyst was transferred. The woman became pregnant and delivered a healthy female.
CONCLUSIONS
The day-7 hatched blastocyst developed from vitrified embryos can be re-vitrified and have pregnancy potential after re-warming.
目的
报告一例经再次冷冻保存的第7天孵化囊胚移植后成功分娩的病例。
方法与结果
一名30岁女性接受了长方案卵巢刺激。获得14个成熟卵母细胞，其中12个受精。第3天，移植了2个分裂期胚胎，但未发生着床。其余胚胎进行玻璃化冷冻。随后，移植了2个玻璃化冷冻的第3天胚胎。该女性怀孕并分娩了一个健康婴儿。随后，又移植了2个玻璃化冷冻的第3天胚胎，但未怀孕。随后，将所有剩余的玻璃化冷冻第3天胚胎进行培养。第5天，未获得囊胚。剩余胚胎继续培养。第7天，获得一个孵化囊胚并再次玻璃化冷冻。随后，移植了再次玻璃化冷冻的第7天孵化囊胚。该女性怀孕并分娩了一个健康女婴。
结论
由玻璃化冷冻胚胎发育而来的第7天孵化囊胚可再次玻璃化冷冻，解冻后具有妊娠潜力。
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