Schwan W R, Waltenbaugh C, Duncan J L
Department of Microbiology-Immunology, Northwestern University, Chicago, IL 60611.
J Immunol Methods. 1990 Feb 9;126(2):247-52. doi: 10.1016/0022-1759(90)90157-q.
A modified solid-phase fluorescence immunoassay was developed using bacterial cells as the solid phase to screen antibodies produced against surface antigens from a clinical isolate of Escherichia coli, strain 1-149. The bacterial solid phase was used to analyze both polyclonal and monoclonal antibodies. The bacterial concentration fluorescence immunoassay (BCFIA) showed up to 50-fold greater sensitivity in bacterial cell detection as compared to ELISA (enzyme-linked immunosorbent assay). Moreover, BCFIA was considerably faster than ELISA with uniform reproducibility. This paper demonstrates the utility of using bacteria and their surface antigens as solid-phase matrices for antibody characterization in a FIA.
开发了一种改良的固相荧光免疫测定法，使用细菌细胞作为固相，以筛选针对大肠杆菌临床分离株1-149表面抗原产生的抗体。细菌固相用于分析多克隆抗体和单克隆抗体。与酶联免疫吸附测定法（ELISA）相比，细菌浓度荧光免疫测定法（BCFIA）在细菌细胞检测中显示出高达50倍的灵敏度。此外，BCFIA比ELISA快得多，具有一致的重现性。本文证明了使用细菌及其表面抗原作为固相基质在荧光免疫测定中表征抗体的实用性。
