采用液相色谱和光电二极管阵列检测法测定肉中的胭脂树橙和降胭脂树橙。

Determination of bixin and norbixin in meat using liquid chromatography and photodiode array detection.

机构信息

Faculty of Veterinary Medicine, Laboratory of Chemical Analysis, Research Group of Veterinary Public Health and Zoonoses, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.

出版信息

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 Jan;26(1):17-24. doi: 10.1080/02652030802322564.

DOI:10.1080/02652030802322564

PMID:19680866

Abstract

The development of an analytical method that enables routine analysis of annatto dye, specifically bixin and norbixin, in meat tissue is described. Liquid-solid extraction was carried out using acetonitrile. Analysis was by HPLC with photodiode array detection using two fixed wavelengths (458 and 486 nm). The possibilities of ion trap mass spectrometry (MS) were also assessed. Method performance characteristics, according to Commission Decision 2002/657/EC, were determined, with recoveries between 99 and 102% and calibration curves being linear in the 0.5-10 mg kg(-1) range. The limit of quantification was 0.5 mg kg(-1).

摘要

本文描述了一种分析方法的开发，该方法可用于常规分析肉类组织中的胭脂树橙染料，特别是胭脂树红和降胭脂树红。采用乙腈进行液-固萃取。分析采用高效液相色谱法，二极管阵列检测，使用两个固定波长（458nm 和 486nm）。还评估了离子阱质谱（MS）的可能性。根据委员会决定 2002/657/EC，确定了方法性能特征，回收率在 99%至 102%之间，校准曲线在 0.5-10mgkg(-1)范围内呈线性。定量限为 0.5mgkg(-1)。