Lancaster F E, Lawrence J F
Food Research Division, Bureau of Chemical Safety, Ottawa, Ontario, Canada.
Food Addit Contam. 1995 Jan-Feb;12(1):9-19. doi: 10.1080/02652039509374274.
Utilizing solvents such as ethanolic aqueous ammonia, petroleum ether, hexane and chloroform, annatto components alpha- and beta-norbixin and alpha- and beta-bixin were extracted from cheese, butter, margarine and hard candy. After transferring the extract into a solution of aqueous acetic acid in methanol, bixin and norbixin were determined quantitatively using high-performance liquid chromatography (HPLC) and an absorbance detector set at 500 nm. Recovery of norbixin from spiked cheese samples averaged 92.6% over a range of 1 to 110 micrograms/g. Commercial cheese samples were found to contain 1.1-68.8 microgram/g total norbixin, and two samples also contained 5.1-5.6 micrograms/g total bixin. Samples of uncoloured butter were spiked with bixin and recovery averaged 93.2% over a range of 0.1 to 445 micrograms/g. Levels of 0.2 microgram/g total bixin and 0.91 microgram/g total norbixin were found in one commercial butter sample; the others contained trace levels of both compounds. Hard candies were prepared in the laboratory and recovery studies conducted. Recovery of norbixin averaged 88%.
利用乙醇氨水溶液、石油醚、己烷和氯仿等溶剂，从奶酪、黄油、人造黄油和硬糖中提取胭脂树素成分α-和β-降胭脂树素以及α-和β-胭脂树素。将提取物转移至甲醇中的乙酸水溶液后，使用高效液相色谱（HPLC）和设置在500nm的吸光度检测器对胭脂树素和降胭脂树素进行定量测定。在1至110微克/克的范围内，加标奶酪样品中降胭脂树素的回收率平均为92.6%。发现市售奶酪样品中总降胭脂树素含量为1.1 - 68.8微克/克，两个样品中还含有5.1 - 5.6微克/克的总胭脂树素。未染色的黄油样品用胭脂树素加标，在0.1至445微克/克的范围内回收率平均为93.2%。在一个市售黄油样品中发现总胭脂树素含量为0.2微克/克，总降胭脂树素含量为0.91微克/克；其他样品中这两种化合物的含量均为痕量。在实验室制备硬糖并进行回收率研究。降胭脂树素的回收率平均为88%。
