Institute for Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany.
Clin Microbiol Infect. 2010 Jun;16(6):633-9. doi: 10.1111/j.1469-0691.2009.02889.x. Epub 2009 Aug 4.
Human bocavirus (HBoV) was recently described as a new member of the Parvoviridae. In order to investigate the suggested association of HBoV with respiratory and gastric disease in infants and young children, sera of 357 paediatric patients hospitalized with infectious and non-infectious diseases were retrospectively analyzed for the presence of HBoV DNA and virus-specific antibodies using quantitative PCR and ELISA, respectively. HBoV seroprevalence was determined to range from 25% in infants younger than 1 year of age to 93% in children aged more than 3 years. Viral loads between 1 x 10(2) and 1.2 x 10(6) geq/mL were observed in 6.7% (20/297) of sera obtained preferentially from young children suffering from infectious diseases. HBoV genomes were furthermore detected in 5% (3/60) of sera collected from individuals with non-infectious illnesses. HBoV DNA was present most frequently in patients with respiratory disease (9.6%). Whereas only 5.2% of patients with upper respiratory tract disease were viraemic, HBoV DNA was found in 14.6% and 10.0% of patients with lower respiratory tract illness and pneumonia, respectively. Acute HBoV infections were also observed in 7.5% of patients with gastroenteritis and in one child with inflammatory bowel disease. None of 77 patients hospitalized for various other infectious diseases (e.g. rash, urinary tract infection, meningitis) displayed viraemia. In 60.9% and 47.8% of DNA-positive children, HBoV-specific IgM and IgG was observed, respectively. The present prospective study provides comprehensive data on the clinical association of acute HBoV infection with respiratory illness and on the seroprevalence of virus-specific antibodies in children.
人博卡病毒（HBoV）最近被描述为细小病毒科的一个新成员。为了研究 HBoV 是否与婴幼儿呼吸道和胃部疾病有关，本研究采用定量 PCR 和 ELISA 分别检测了 357 例因感染性和非感染性疾病住院的儿科患者血清中 HBoV DNA 和病毒特异性抗体。结果显示，HBoV 血清阳性率在 1 岁以下婴儿中为 25%，3 岁以上儿童中为 93%。在 297 例主要来自患有感染性疾病的年幼儿童的血清中，有 6.7%（20/297）的病毒载量在 1×10^2 到 1.2×10^6 geq/mL 之间。此外，还在 5%（3/60）的非感染性疾病患者血清中检测到 HBoV 基因组。HBoV DNA 主要存在于呼吸道疾病患者（9.6%）中。仅有 5.2%的上呼吸道疾病患者存在病毒血症，而下呼吸道疾病和肺炎患者的病毒血症发生率分别为 14.6%和 10.0%。在 7.5%的肠胃炎患者和 1 例炎症性肠病患者中也观察到急性 HBoV 感染。在因各种其他感染性疾病（如皮疹、尿路感染、脑膜炎）住院的 77 例患者中，均未发现病毒血症。在 DNA 阳性的儿童中，分别有 60.9%和 47.8%检测到 HBoV 特异性 IgM 和 IgG。本前瞻性研究提供了全面的数据，说明急性 HBoV 感染与呼吸道疾病有关，以及儿童中病毒特异性抗体的血清阳性率。
