Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, Gwangju City, South Korea.
J Ethnopharmacol. 2009 Oct 29;126(1):90-5. doi: 10.1016/j.jep.2009.08.004. Epub 2009 Aug 13.
Geranium sibiricum (Geraniaceae) Linne (GSL) is used to heal various disorders of the diarrhea and the intestinal inflammation as an herbal agent in East Asia.
The purpose of the present study is to determine whether the ethanol (EtOH) extract of GSL regulates the inflammatory reaction stimulated by phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) in human mast cells (HMC-1).
Western blot was used for activation of mitogen activated protein kinase (MAPK), transcription factors, induced nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 proteins. EMSA was for DNA binding activity. RT-PCR was used for gene expression.
EtOH extract of GSL (EGS) inhibits the expression of extracellular signal-regulated kinase (ERK), one of a MAPK, nuclear transcription factors involving nuclear factor (NF)-kappaB and Activator protein (AP)-1, COX-2 and iNOS. The results indicated that EGS decreased gene expression of interleukin (IL)-1beta and COX-2 in PMACI stimulated HMC-1 cells.
Hence, we speculate that EGS can use as a potent anti-inflammatory agent for inflammatory allergic diseases.
Geranium sibiricum（牻牛儿苗科）Linne（GSL）被用作治疗东亚地区腹泻和肠道炎症等各种疾病的草药。
本研究的目的是确定 GSL 的乙醇（EtOH）提取物是否可调节佛波醇 12-肉豆蔻酸 13-醋酸酯加钙离子载体 A23187（PMACI）刺激的人肥大细胞（HMC-1）中的炎症反应。
采用 Western blot 检测细胞分裂原激活蛋白激酶（MAPK）、转录因子、诱导型一氧化氮合酶（iNOS）和环氧化酶（COX）-2 蛋白的激活。采用 EMSA 检测 DNA 结合活性。采用 RT-PCR 检测基因表达。
GSL 的 EtOH 提取物（EGS）抑制细胞外信号调节激酶（ERK）的表达，ERK 是 MAPK 之一，还涉及核转录因子核因子（NF）-κB 和激活蛋白（AP）-1、COX-2 和 iNOS。结果表明，EGS 可降低 PMACI 刺激的 HMC-1 细胞中白细胞介素（IL）-1β和 COX-2 的基因表达。
因此，我们推测 EGS 可作为治疗炎症性过敏疾病的有效抗炎药物。
