Chien Shih-Chang, Young Paul H, Hsu Yi-Jou, Chen Chun-Houh, Tien Yin-Jing, Shiu Shang-Ying, Li Tzu-Hsuan, Yang Chi-Wen, Marimuthu Palanisamy, Tsai Leo Feng-Liang, Yang Wen-Chin
Agricultural Biotechnology Research Center, Academia Sinica, Taiwan, ROC.
Phytochemistry. 2009 Jul;70(10):1246-54. doi: 10.1016/j.phytochem.2009.07.011. Epub 2009 Aug 14.
Bidens pilosa L. var. radiata (BPR), B. pilosa L. var. pilosa (BPP), and B. pilosa L. var. minor (BPM) are common variants of a plant often used as a folk remedy for diabetes in Taiwan. However, the three variants are often misidentified and little is known about their relative anti-diabetic efficacy and chemical composition. In this paper, we have first developed a method based on GC-MS and cluster analysis with visualization to assist in rapidly determining the taxonomy of these three Bidens variants. GC-MS was used to determine the chemical compositions of supercritical extracts, and differences and similarities in the variants were determined by hierarchical cluster analysis. Next, the HPLC profiles of the methanol crude extracts in the Bidens plants and evaluated anti-diabetic effects of methanol crude extracts were compared, as well as three polyacetylenic compounds of the Bidens plants using db/db mice. Single-dose and long-term experiments showed that the BPR extract had higher glucose-lowering and insulin-releasing activities than extracts from the other two variants, and that cytopiloyne was the most effective pure compound among the three polyacetylenic compounds. BPR extract and cytopiloyne also significantly reduced the percentage of the glycosylated hemoglobin A1c in db/db mice. Besides, both animal studies and HPLC analysis demonstrated a good correlation between anti-diabetic efficacy of the Bidens extracts and the particular polyacetylenes present.
咸丰草变种咸丰草（BPR）、咸丰草原变种（BPP）和咸丰草小叶变种（BPM）是一种常见植物的变种，在台湾常被用作治疗糖尿病的民间药物。然而，这三个变种常被误认，人们对它们相对的抗糖尿病功效和化学成分知之甚少。在本文中，我们首先开发了一种基于气相色谱 - 质谱联用（GC-MS）和可视化聚类分析的方法，以协助快速确定这三种咸丰草变种的分类。利用GC-MS测定超临界提取物的化学成分，并通过层次聚类分析确定变种之间的异同。接下来，比较了咸丰草植物甲醇粗提物的高效液相色谱（HPLC）图谱，并评估了甲醇粗提物的抗糖尿病作用，以及使用db/db小鼠对咸丰草植物的三种聚乙炔化合物进行了研究。单剂量和长期实验表明，BPR提取物比其他两种变种的提取物具有更高的降血糖和胰岛素释放活性，并且在三种聚乙炔化合物中，刺孢囊菌素是最有效的纯化合物。BPR提取物和刺孢囊菌素还显著降低了db/db小鼠糖化血红蛋白A1c的百分比。此外，动物研究和HPLC分析均表明，咸丰草提取物的抗糖尿病功效与其中特定的聚乙炔化合物之间存在良好的相关性。
