Sacks D L, Esser K M, Sher A
J Exp Med. 1982 Apr 1;155(4):1108-19. doi: 10.1084/jem.155.4.1108.
Anti-idiotypic (anti-Id) antibodies were raised against three protective monoclonal antibodies, each with specificity for the variable antigen type (VAT) of a clone of Trypanosoma rhodesiense. The IgG1 fractions of each were pooled and administered to BALB/c mice 3-4 wk before homologous challenge. The course of primary parasitemia was altered in 19 of 30 anti-Id-treated animals. The immunity was manifested as either: (a) complete protection, (b) reduced parasitemia, or (c) selection against parasites bearing the original VAT. The three idiotypes (Id) were found in variable levels in serum during the course of infection in control animals. However, in all anti-Id-treated mice that displayed immunity, one Id in particular (7H11) was detectable much earlier in infection and in higher levels than in control mice or anti-Id-treated, nonimmune mice. Six of nine mice treated with the anti-7H11 Id alone also displayed immunity, manifested in this case exclusively as selection against parasites bearing the original VAT. The effect was again associated with the more rapid appearance of the Id after infection. Specificity of the anti-Id-induced immunity was supported by the failure of anti-7H11 Id treatment to alter the course of infection with a heterologous clone of T. rhodesiense. To our knowledge, this is the first report of the antigen-independent induction of antimicrobial immunity using anti-Id antibodies.
制备了针对三种保护性单克隆抗体的抗独特型（抗Id）抗体，每种单克隆抗体对罗德西亚锥虫一个克隆的可变抗原类型（VAT）具有特异性。将每种抗体的IgG1组分混合，并在同源攻击前3 - 4周给予BALB/c小鼠。在30只接受抗Id治疗的动物中，有19只动物的初次寄生虫血症病程发生了改变。这种免疫表现为以下几种情况：（a）完全保护，（b）寄生虫血症降低，或（c）对携带原始VAT的寄生虫进行选择淘汰。在对照动物感染过程中，三种独特型（Id）在血清中的水平各不相同。然而，在所有表现出免疫的抗Id治疗小鼠中，特别是一种Id（7H11）在感染早期就能检测到，且水平高于对照小鼠或接受抗Id治疗但无免疫反应的小鼠。仅用抗7H11 Id治疗的9只小鼠中有6只也表现出免疫，在这种情况下仅表现为对携带原始VAT的寄生虫进行选择淘汰。这种效应同样与感染后Id更快出现有关。用抗7H11 Id治疗未能改变罗德西亚锥虫异源克隆的感染病程，这支持了抗Id诱导免疫的特异性。据我们所知，这是首次使用抗Id抗体进行不依赖抗原的抗微生物免疫诱导的报告。
