Microbial Biotechnology Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, 14155-6455, Tehran, Iran.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2010 Jul;60(Pt 7):1504-1509. doi: 10.1099/ijs.0.015339-0. Epub 2009 Aug 14.
A novel streptomycete, designated strain HM 35(T), was isolated from soil in Isfahan city, Iran. Strain HM 35(T) produced a branched substrate mycelium and aerial hyphae that developed into short, compact, spiral spore chains with grey rugose spores at the tips of the aerial hyphae. On some media, these spirals coalesced into dark masses of spores with age. Whole-cell hydrolysates of strain HM 35(T) contained LL-diaminopimelic acid, glucose and ribose. Phospholipids detected were phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylinositol mannosides, hydroxy-phosphatidylethanolamine, lyso-phosphatidylethanolamine and hydroxy-lyso-phosphatidylethanolamine. MK-9(H(4)), MK-9(H(6)) and MK-9(H(8)) were the predominant menaquinones. The major fatty acids were iso- and anteiso-branched components. The chemotaxonomic characteristics of the novel isolate matched those described for members of the genus Streptomyces. Based on 16S rRNA gene sequence analysis, strain HM 35(T) showed highest similarity to Streptomyces rapamycinicus NRRL 5491(T) (99.2 %), Streptomyces violaceusniger DSM 40563(T) (99.1 %), Streptomyces javensis DSM 41764(T) (99.1 %) and Streptomyces yogyakartensis DSM 41766(T) (99.1 %). The novel strain formed a distinct monophyletic line within the 16S rRNA gene sequence tree. The level of DNA-DNA relatedness between strain HM 35(T) and the type strain of S. rapamycinicus was 72.7 %. Strain HM 35(T) showed the typical morphology found among members of the S. violaceusniger/Streptomyces hygroscopicus group but could be clearly differentiated from closely related species based on other phenotypic markers. Phenotypic and genotypic data thus indicate that strain HM 35(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces iranensis is proposed. The type strain is HM 35(T) (=DSM 41954(T)=CCUG 57623(T)).
一株新型链霉菌，命名为 HM 35(T)，从伊朗伊斯法罕市的土壤中分离得到。HM 35(T)菌株产生分支的基质菌丝和气生菌丝，气生菌丝发育成短而紧凑的螺旋孢子链，气生菌丝的顶端有灰色粗糙的孢子。在一些培养基上，这些螺旋随着年龄的增长而合并成黑色的孢子团。HM 35(T)菌株的全细胞水解物含有 LL-二氨基庚二酸、葡萄糖和核糖。检测到的磷脂有磷脂酰甘油、磷脂酰肌醇、磷脂酰乙醇胺、磷脂酰肌醇甘露糖苷、羟基磷脂酰乙醇胺、溶菌磷脂酰乙醇胺和羟基溶菌磷脂酰乙醇胺。MK-9(H(4))、MK-9(H(6))和 MK-9(H(8))是主要的menaquinones。主要脂肪酸是异和前异分支成分。新型分离株的化学分类特征与链霉菌属的成员描述相符。基于 16S rRNA 基因序列分析，HM 35(T)菌株与雷帕霉素链霉菌 NRRL 5491(T)（99.2%）、紫色链霉菌黑色变种 DSM 40563(T)（99.1%）、雅加羥链霉菌 DSM 41764(T)（99.1%）和雅加羥链霉菌 DSM 41766(T)（99.1%）最为相似。新型菌株在 16S rRNA 基因序列树中形成一个独特的单系。HM 35(T)菌株与雷帕霉素链霉菌模式株之间的 DNA-DNA 同源性水平为 72.7%。HM 35(T)菌株表现出紫色链霉菌/链霉菌 hygroscopicus 组中成员的典型形态，但可以通过其他表型标记清楚地区分与密切相关的物种。表型和基因型数据表明，HM 35(T)菌株代表链霉菌属的一个新种，建议将其命名为伊朗链霉菌。模式菌株为 HM 35(T)=DSM 41954(T)=CCUG 57623(T)。
