Zhang Qiufang, Bai Quan, Yuan Yang, Liu Ping, Qiao Jie
Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Haidian District, Beijing, China 100083.
J Androl. 2010 Mar-Apr;31(2):215-20. doi: 10.2164/jandrol.109.007609. Epub 2009 Aug 14.
The proposed hypothesis for this study was that seminal testosterone/estradiol levels and/or their ratios may be a good indicator for predicting normal spermatogenesis. The concentrations of estradiol and testosterone in seminal fluid were measured using competitive immunoassay techniques in specimens collected from 192 infertile patients and 103 normospermic men. Infertile patients were subdivided into three groups according to their semen analysis results and testicular biopsy: oligozoospermia, obstructive azoospermia (OA), and nonobstructive azoospermia (NOA). Results showed that seminal testosterone levels in the infertile groups were lower than in the normospermic individuals (P < .01), whereas seminal estradiol levels in the OA group were significantly higher than those in normospermic and NOA groups (P < .01). Testosterone/estradiol ratios in the seminal plasma from the infertile groups were significantly lower than that in the normospermic group (P < .01). However, seminal estradiol levels among normospermic and NOA groups showed no significant differences. These results suggest that the local balance between androgen and estrogen, or their ratios, may play an important role in maintaining normal spermatogenesis. Also, decreased seminal testosterone/estradiol ratio may be a good indicator for identifying the absence of sperm production in NOA patients.
本研究提出的假设是，精液中的睾酮/雌二醇水平和/或其比值可能是预测正常精子发生的良好指标。采用竞争性免疫分析技术，对192例不育患者和103例精子正常的男性采集的标本中的精液雌二醇和睾酮浓度进行了测定。不育患者根据精液分析结果和睾丸活检分为三组：少精子症、梗阻性无精子症（OA）和非梗阻性无精子症（NOA）。结果显示，不育组的精液睾酮水平低于精子正常的个体（P <.01），而OA组的精液雌二醇水平显著高于精子正常组和NOA组（P <.01）。不育组精液中睾酮/雌二醇比值显著低于精子正常组（P <.01）。然而，精子正常组和NOA组之间的精液雌二醇水平无显著差异。这些结果表明，雄激素和雌激素之间的局部平衡或其比值可能在维持正常精子发生中起重要作用。此外，精液睾酮/雌二醇比值降低可能是识别NOA患者无精子生成的良好指标。
