Santos Ethienne Lobato dos, Tamegão-Lopes Bruna, Machado Luiz Fernando Almeida, Ishak Marluísa de Oliveira Guimarães, Ishak Ricardo, Lemos José Alexandre Rodrigues de, Vallinoto Antonio Carlos Rosário
Laboratório Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA.
Rev Soc Bras Med Trop. 2009 May-Jun;42(3):271-6. doi: 10.1590/s0037-86822009000300007.
This study aimed to perform molecular characterization on the human T-cell lymphotropic virus (HTLV) infecting blood donors attended at the Hematology and Hemotherapy Center-Foundation of Pará. DNA samples from 79 HTLV-seropositive individuals were analyzed by means of the polymerase chain reaction on the pX, env and 5'LTR genomic regions; restriction fragment length polymorphism analysis; and sequencing of the 5'LTR region with subsequent phylogenetic analysis. From this, the HTLV types and subtypes circulating in the study population were defined. There was higher prevalence of HTLV-1 (71%) than of HTLV-2 (29%). HTLV-1 samples were classified as belonging to the Cosmopolitan subtype, Transcontinental subgroup; and the HTLV-2 samples as HTLV-2c. Analysis on the restriction fragment length polymorphisms of the env region and sequencing of the 5'LTR region identified a sample of HTLV-2b, for the first time in the Brazilian Amazon region. This emphasizes the need for ongoing molecular studies in this region, in order to have better understanding of the epidemiology of HTLV transmission in the population, and to enable epidemiological surveillance of the emergence of new types and subtypes.
本研究旨在对感染帕拉州血液学与血液治疗中心基金会供血者的人类嗜T淋巴细胞病毒（HTLV）进行分子特征分析。通过对pX、env和5'LTR基因组区域进行聚合酶链反应、限制性片段长度多态性分析以及对5'LTR区域进行测序并随后进行系统发育分析，对79名HTLV血清阳性个体的DNA样本进行了分析。由此确定了研究人群中传播的HTLV类型和亚型。HTLV-1的流行率（71%）高于HTLV-2（29%）。HTLV-1样本被归类为属于世界主义亚型、跨大陆亚组；HTLV-2样本被归类为HTLV-2c。对env区域的限制性片段长度多态性分析和5'LTR区域的测序首次在巴西亚马逊地区鉴定出一份HTLV-2b样本。这强调了该地区持续进行分子研究的必要性，以便更好地了解HTLV在人群中的传播流行病学，并对新型和亚型的出现进行流行病学监测。
