Lima Junior Manoel Sebastião da Costa, Andreotti Renato, Dorval Maria Elizabeth Moraes Cavalheiros, Oshiro Elisa Teruya, Oliveira Alessandra Gutierrez de, Matos Maria de Fatima Cepa
Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS.
Rev Soc Bras Med Trop. 2009 May-Jun;42(3):303-8. doi: 10.1590/s0037-86822009000300012.
Leishmaniases are endemic zoonoses in the State of Mato Grosso do Sul. Their etiological agents in this region of Brazil are Leishmania (Leishmania) chagasi, Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis. The polymerase chain reaction (PCR) is a tool with high specificity and sensitivity for identifying Leishmania species. This study examined 39 cryopreserved isolates of Leishmania that had been collected by bone marrow aspiration and/or lesion biopsy, depending on the clinical suspicion. The isolates were subjected to DNA extraction and PCR using the following primers: RV1/RV2 for identifying Leishmania (Leishmania) chagasi, a1/a2 for Leishmania (Leishmania) amazonensis and b1/b2 for Leishmania (Viannia) braziliensis.Leishmania (Leishmania) chagasi was the only species identified in the 37 cases of visceral leishmaniasis.Leishmania (Leishmania) amazonensis was identified in two isolates from patients with a diagnosis of cutaneous leishmaniasis. The results obtained confirm that it is possible to use these three pairs of primers as a tool for characterizing Leishmania isolates.
利什曼病是南马托格罗索州的地方性人畜共患病。在巴西的这个地区，其病原体为恰加斯利什曼原虫（Leishmania (Leishmania) chagasi）、亚马逊利什曼原虫（Leishmania (Leishmania) amazonensis）和巴西利什曼原虫（Leishmania (Viannia) braziliensis）。聚合酶链反应（PCR）是一种用于鉴定利什曼原虫种类的具有高特异性和敏感性的工具。本研究检测了39株通过骨髓穿刺和/或病变活检采集的利什曼原虫冷冻保存菌株，具体采集方式取决于临床怀疑情况。对这些菌株进行DNA提取，并使用以下引物进行PCR：用于鉴定恰加斯利什曼原虫的RV1/RV2、用于亚马逊利什曼原虫的a1/a2以及用于巴西利什曼原虫的b1/b2。恰加斯利什曼原虫是在37例内脏利什曼病病例中唯一鉴定出的物种。在两名诊断为皮肤利什曼病患者的分离株中鉴定出了亚马逊利什曼原虫。获得的结果证实，这三对引物可作为鉴定利什曼原虫分离株的工具。
