斑马鱼减数分裂的免疫荧光显微镜研究。

Immunofluorescent microscopic study of meiosis in zebrafish.

作者信息

Kochakpour Nazafarin

机构信息

Department of Biology, York University, Toronto, Ontario, Canada.

出版信息

Methods Mol Biol. 2009;558:251-60. doi: 10.1007/978-1-60761-103-5_15.

DOI:10.1007/978-1-60761-103-5_15

PMID:19685329

Abstract

In recent years, zebrafish (Danio rerio) has been used as a model vertebrate organism for studies of human disease, development, and genetics. This chapter describes detailed methods for the preparation of whole-mount meiotic oocytes and spermatocytes as well as cryostat sectioning of ovaries and testes for immunofluorescence microscopy studies of zebrafish.

摘要

近年来，斑马鱼（Danio rerio）已被用作模式脊椎动物，用于人类疾病、发育和遗传学研究。本章介绍了制备整装减数分裂卵母细胞和精母细胞的详细方法，以及用于斑马鱼免疫荧光显微镜研究的卵巢和睾丸的冰冻切片方法。