Technische Universität Darmstadt, Fachbereich Material-u. Geowissenschaften, Fachgebiet Chemische Analytik, Petersenstrasse 23, D-64287 Darmstadt, Germany.
Macromol Biosci. 2010 Jan 11;10(1):28-32. doi: 10.1002/mabi.200900198.
In this work, we describe the direct covalent attachment of protein recognition elements (biotin) with the carboxyl groups present on the walls of polyimide nanochannels. Subsequently, these biotinylated channels were used for the bio-specific sensing of protein analytes. Moreover, surface charge of these asymmetric nanochannels was reversed from negative to positive via the conversion of carboxyl groups into terminated amino groups. The negatively charge (carboxylated) and positively charged (aminated) channels were further used for the electrochemical sensing of bovine serum albumin (BSA, pI = 4.7). These biorecognition events were assessed from the changes in the ionic current flowing through the nanochannel.
在这项工作中，我们描述了蛋白质识别元件（生物素）与聚酰亚胺纳米通道壁上存在的羧基之间的直接共价连接。随后，这些生物素化的通道被用于生物特异性蛋白质分析物的传感。此外，通过将羧基转化为端氨基，这些不对称纳米通道的表面电荷从负变为正。负电荷（羧基化）和正电荷（氨基化）通道进一步用于牛血清白蛋白（BSA，pI = 4.7）的电化学传感。这些生物识别事件是通过流经纳米通道的离子电流的变化来评估的。
