Department of Physiology, Iran University of Medical Sciences, Tehran, Iran.
Fundam Clin Pharmacol. 2010 Apr;24(2):189-98. doi: 10.1111/j.1472-8206.2009.00743.x. Epub 2009 Aug 17.
Four pre-medication drugs are used to relieve pain, allay anxiety, reduce secretion and enhance hypnosis, were evaluated for their effects on ischemia reperfusion (I/R) injury which is one of the major complications of vascular and transplantation surgery. Right kidney was removed from female rats (210-250 g) 3 weeks before surgical procedure. Different doses of morphine (0.5, 2 and 5 mg/kg), promethazine (1, 2 and 5 mg/kg), atropine (0.1, 0.3 and 0.5 mg/kg) and alprazolam (0.08, 0.32 and 0.64 mg/kg) were administered subcutaneously 30 min before left renal artery occlusion and 6 h reperfusion. Left kidneys were processed for histological evaluations. Creatinine and BUN were measured in serum samples. Morphine, promethazine, atropine and alprazolam at all evaluated doses significantly decreased serum creatinine and BUN levels and histopathological scores. The effects of promethazine (1 mg/kg) and all doses of alprazolam were more potent than other pre-medication drugs and doses. This study suggested a protective effect of these pre-medication drugs on I/R injury. Although obvious studies are required, these findings may lead to effective therapies against I/R injury.
四种术前用药用于缓解疼痛、减轻焦虑、减少分泌和增强催眠作用，评估它们对缺血再灌注（I/R）损伤的影响，这是血管和移植手术的主要并发症之一。在手术前 3 周，从雌性大鼠（210-250 g）中取出右肾。在左肾动脉闭塞和 6 小时再灌注前 30 分钟，皮下给予不同剂量的吗啡（0.5、2 和 5 mg/kg）、异丙嗪（1、2 和 5 mg/kg）、阿托品（0.1、0.3 和 0.5 mg/kg）和阿普唑仑（0.08、0.32 和 0.64 mg/kg）。处理左肾进行组织学评估。测量血清样本中的肌酐和 BUN。吗啡、异丙嗪、阿托品和阿普唑仑在所有评估剂量下均显著降低血清肌酐和 BUN 水平以及组织病理学评分。异丙嗪（1 mg/kg）和所有剂量的阿普唑仑的作用比其他术前用药和剂量更强。这项研究表明这些术前用药对 I/R 损伤具有保护作用。尽管需要进行更明显的研究，但这些发现可能会导致针对 I/R 损伤的有效治疗方法。
