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DySCo：使用双色单分子追踪技术对膜蛋白关联进行定量分析。

DySCo: quantitating associations of membrane proteins using two-color single-molecule tracking.

作者信息

Dunne Paul D, Fernandes Ricardo A, McColl James, Yoon Ji Won, James John R, Davis Simon J, Klenerman David

出版信息

Biophys J. 2009 Aug 19;97(4):L5-7. doi: 10.1016/j.bpj.2009.05.046.

DOI:10.1016/j.bpj.2009.05.046
PMID:19686638
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2726305/
Abstract

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.

摘要

我们提出了一种称为动态单分子共定位的通用方法，用于定量分析用不同自发荧光蛋白标记的单细胞表面分子之间的关联。这种新的定量方法的主要优点是，除了稳定的相互作用外，它还能够测量非组成性关联，例如由细胞骨架诱导的关联，并且适用于分子数量较少的情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a1/2726305/736cb3fd94cd/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a1/2726305/7c9af7f3a084/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a1/2726305/736cb3fd94cd/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a1/2726305/7c9af7f3a084/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a1/2726305/736cb3fd94cd/gr2.jpg

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2
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3
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4
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Front Immunol. 2018 Sep 25;9:2152. doi: 10.3389/fimmu.2018.02152. eCollection 2018.
5
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