Lee Juyeon, Moon Changjong, Kim Jihoon, Jung Chanwoo, Lee Keun-Hwa, Joo Hong-Gu, Ahn Meejung, Shin Taekyun
Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Korea.
J Vet Sci. 2009 Sep;10(3):177-80. doi: 10.4142/jvs.2009.10.3.177.
The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.
通过免疫组织化学方法对感染副结核病（约翰氏病）的牛肠道中半乳糖凝集素-3的存在情况进行定量分析，以确定半乳糖凝集素-3是否参与了与该疾病相关的肉芽组织形成。使用齐-尼氏染色法对鸟分枝杆菌副结核亚种感染进行组织化学确认，并通过rpoB DNA测序进行分子诊断。在包括回肠在内的受影响组织的肉芽肿病变中的大多数炎性细胞（可能是巨噬细胞）中检测到了半乳糖凝集素-3。这些发现表明，半乳糖凝集素-3可能通过细胞黏附和抗凋亡机制与牛副结核病中慢性肉芽组织的形成有关。
