Okuni Julius Boniface, Reinacher Manfred, Loukopoulos Panayiotis, Ojok Lonzy
College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P.O. Box 7062, Kampala, Uganda.
Trop Anim Health Prod. 2013 Jun;45(5):1197-202. doi: 10.1007/s11250-012-0346-3. Epub 2012 Dec 30.
This study was conducted to determine the prevalence and characteristics of Johne's disease (JD) lesions in Ugandan cattle slaughtered at two of the main abattoirs in Kampala. Ileocaecal junction and the associated lymph nodes of 1,022 cattle were examined for gross and microscopic lesions, followed by Ziehl Neelsen staining of the tissues bearing lesions. Confirmation of Mycobacterium avium subsp. paratuberculosis infection was done in some of the tissues using culture and IS900 PCR. The lesions were then described, characterised and tabulated. Characteristic Johne's disease granulomas were found in 4.7% of the samples examined, derived from Zebu, Ankole longhorn, Friesian breeds of cattle and their crosses. Lesions were found both in the lymph nodes and ileocaecal junction mucosa. The lesions tended to be more severe in the lymph node than in the mucosa. There were also some unique and atypical lesions found in association with Johne's disease granulomas. The diagnostic values of various gross lesions and criteria of lesion classifications and diagnosis are revisited and discussed based on the findings of this study. The prevalence of Johne's disease lesions among slaughtered cattle in Kampala's two abattoirs indicates that the disease is well established in the cattle population in the country. The diverse manifestations in lesions of JD need to be considered when making histological diagnosis in tissues where the disease is suspected.
本研究旨在确定在坎帕拉两个主要屠宰场宰杀的乌干达牛中，约内氏病（JD）病变的患病率和特征。对1022头牛的回盲交界处及相关淋巴结进行大体和显微镜检查，随后对有病变的组织进行齐-尼氏染色。部分组织采用培养法和IS900 PCR法对副结核分枝杆菌亚种感染进行确诊。然后对病变进行描述、特征分析并制成表格。在所检查的样本中，4.7%发现了典型的约内氏病肉芽肿，这些样本来自瘤牛、安科拉长角牛、弗里斯兰牛品种及其杂交品种。病变见于淋巴结和回盲交界黏膜。淋巴结中的病变往往比黏膜中的更严重。还发现了一些与约内氏病肉芽肿相关的独特和非典型病变。基于本研究结果，重新审视并讨论了各种大体病变的诊断价值以及病变分类和诊断标准。坎帕拉两个屠宰场宰杀牛中约内氏病病变的患病率表明，该病在该国牛群中已广泛存在。在疑似该病的组织进行组织学诊断时，需要考虑约内氏病病变的多种表现形式。
