Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, PR China.
J Exp Bot. 2010 Jun;61(10):2647-68. doi: 10.1093/jxb/erq104. Epub 2010 Apr 27.
Arabinogalactan proteins (AGPs) comprise a family of hydroxyproline-rich glycoproteins that are implicated in plant growth and development. In this study, 69 AGPs are identified from the rice genome, including 13 classical AGPs, 15 arabinogalactan (AG) peptides, three non-classical AGPs, three early nodulin-like AGPs (eNod-like AGPs), eight non-specific lipid transfer protein-like AGPs (nsLTP-like AGPs), and 27 fasciclin-like AGPs (FLAs). The results from expressed sequence tags, microarrays, and massively parallel signature sequencing tags are used to analyse the expression of AGP-encoding genes, which is confirmed by real-time PCR. The results reveal that several rice AGP-encoding genes are predominantly expressed in anthers and display differential expression patterns in response to abscisic acid, gibberellic acid, and abiotic stresses. Based on the results obtained from this analysis, an attempt has been made to link the protein structures and expression patterns of rice AGP-encoding genes to their functions. Taken together, the genome-wide identification and expression analysis of the rice AGP gene family might facilitate further functional studies of rice AGPs.
阿拉伯半乳糖蛋白（AGPs）是一类富含羟脯氨酸的糖蛋白，参与植物生长和发育。本研究从水稻基因组中鉴定出 69 种 AGP，包括 13 种经典 AGP、15 种阿拉伯半乳糖（AG）肽、3 种非经典 AGP、3 种早期结瘤素样 AGP（eNod-like AGP）、8 种非特异性脂质转移蛋白样 AGP（nsLTP-like AGP）和 27 种纤维连接蛋白样 AGP（FLA）。表达序列标签、微阵列和大规模平行签名测序标签的结果用于分析 AGP 编码基因的表达，实时 PCR 对其进行了验证。结果表明，几种水稻 AGP 编码基因主要在花药中表达，并对脱落酸、赤霉素和非生物胁迫表现出不同的表达模式。基于该分析的结果，尝试将水稻 AGP 编码基因的蛋白质结构和表达模式与其功能联系起来。总的来说，水稻 AGP 基因家族的全基因组鉴定和表达分析可能有助于进一步研究水稻 AGP 的功能。
