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水稻（Oryza sativa L.）肽转运蛋白家族的基因组调查、特征描述和表达谱分析。

Genomic survey, characterization and expression profile analysis of the peptide transporter family in rice (Oryza sativa L.).

机构信息

National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research Wuhan, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

BMC Plant Biol. 2010 May 20;10:92. doi: 10.1186/1471-2229-10-92.

DOI:10.1186/1471-2229-10-92
PMID:20487558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3017762/
Abstract

BACKGROUND

Peptide transporter (PTR) family whose member can transport di-/tripeptides and nitrate is important for plant growth and development. Although the rice (Oryza sativa L.) genome has been sequenced for a few years, a genomic survey, characterization and expression profile analysis of the PTR family in this species has not been reported.

RESULTS

In this study, we report a comprehensive identification, characterization, phylogenetic and evolutionary analysis of 84 PTR family members in rice (OsPTR) as well as their whole-life expression patterns. Chromosomal distribution and sequence analysis indicate that nearly 70% of OsPTR members are involved in the tandem and segmental duplication events. It suggests that genome duplication might be a major mechanism for expansion of this family. Highly conserved motifs were identified in most of the OsPTR members. Meanwhile, expression profile of OsPTR genes has been analyzed by using Affymetrix rice microarray and real-time PCR in two elite hybrid rice parents, Minghui 63 and Zhenshan 97. Seven genes are found to exhibit either preferential or tissue-specific expression during different development stages of rice. Under phytohormone (NAA, GA3 and KT) and light/dark treatments, 14 and 17 OsPTR genes are differentially expressed respectively. Ka/Ks analysis of the paralogous OsPTR genes indicates that purifying selection plays an important role in function maintenance of this family.

CONCLUSION

These investigations add to our understanding of the importance of OsPTR family members and provide useful reference for selecting candidate genes for functional validation studies of this family in rice.

摘要

背景

肽转运蛋白（PTR）家族的成员可以转运二肽/三肽和硝酸盐，对植物的生长和发育很重要。尽管水稻（Oryza sativa L.）的基因组已经测序了几年，但该物种的 PTR 家族的基因组调查、特征分析和表达谱分析尚未报道。

结果

在这项研究中，我们报告了水稻（OsPTR）84 个 PTR 家族成员的全面鉴定、特征分析、系统进化和表达模式分析。染色体分布和序列分析表明，近 70%的 OsPTR 成员涉及串联和片段重复事件。这表明基因组复制可能是该家族扩张的主要机制。大多数 OsPTR 成员都具有高度保守的基序。同时，利用 Affymetrix 水稻微阵列和实时 PCR 分析了两个优良杂交水稻亲本明恢 63 和珍汕 97 中的 OsPTR 基因的表达谱。发现 7 个基因在水稻不同发育阶段表现出优先或组织特异性表达。在植物激素（NAA、GA3 和 KT）和光照/黑暗处理下，分别有 14 个和 17 个 OsPTR 基因差异表达。直系同源 OsPTR 基因的 Ka/Ks 分析表明，纯化选择在该家族功能维持中起着重要作用。

结论

这些研究增加了我们对 OsPTR 家族成员重要性的理解，并为在水稻中选择该家族功能验证研究的候选基因提供了有用的参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/910a/3017762/5442784ecba0/1471-2229-10-92-9.jpg
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本文引用的文献

1
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2
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Mol Plant. 2008 Sep;1(5):715-9. doi: 10.1093/mp/ssn043. Epub 2008 Aug 13.
3
Mobyle: a new full web bioinformatics framework.Mobyle：一个全新的全网络生物信息学框架。
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Int J Mol Sci. 2022 Jun 15;23(12):6663. doi: 10.3390/ijms23126663.
4
Comparative transcriptome and metabolome profiling reveal molecular mechanisms underlying OsDRAP1-mediated salt tolerance in rice.比较转录组和代谢组谱分析揭示了 OsDRAP1 介导的水稻耐盐性的分子机制。
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5
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6
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BMC Plant Biol. 2020 May 6;20(1):193. doi: 10.1186/s12870-020-02419-y.
7
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4
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8
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